reversible disassembly of the actin cytoskeleton improves the survival rate and developmental competence of cryopreserved mouse oocytes可逆的拆卸的肌动蛋白细胞骨架提高存活率和低温贮藏小鼠卵母细胞的发育能力.pdfVIP

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2017-09-11 发布于上海
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reversible disassembly of the actin cytoskeleton improves the survival rate and developmental competence of cryopreserved mouse oocytes可逆的拆卸的肌动蛋白细胞骨架提高存活率和低温贮藏小鼠卵母细胞的发育能力.pdf

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reversible disassembly of the actin cytoskeleton improves the survival rate and developmental competence of cryopreserved mouse oocytes可逆的拆卸的肌动蛋白细胞骨架提高存活率和低温贮藏小鼠卵母细胞的发育能力

Reversible Disassembly of the Actin Cytoskeleton Improves the Survival Rate and Developmental Competence of Cryopreserved Mouse Oocytes 1 2 4 3,4,5 Basarab G. Hosu , Steven F. Mullen *, John K. Critser , Gabor Forgacs 1 Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, United States of America, 2 21st Century Medicine, Fontana, California, United States of America, 3 Department of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America, 4 Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, United States of America, 5 Department of Physics, University of Missouri, Columbia, Missouri, United States of America Abstract Effective cryopreservation of oocytes is critically needed in many areas of human reproductive medicine and basic science, such as stem cell research. Currently, oocyte cryopreservation has a low success rate. The goal of this study was to understand the mechanisms associated with oocyte cryopreservation through biophysical means using a mouse model. Specifically, we experimentally investigated the biomechanical properties of the ooplasm prior and after cryopreservation as well as the consequences of reversible dismantling of the F-actin network in mouse oocytes prior to freezing. The study was complemented with the evaluation of post-thaw developmental competence of oocytes after in vitro fertilization. Our results show that the freezing-thawing process markedly alters the physiological viscoelastic properties of the actin cytoskeleton. The reversible depolymerization of the F-actin network prior to freezing preserves normal ooplasm