repulsive forces between looping chromosomes induce entropy-driven segregation排斥力循环染色体之间引起熵驱动隔离.pdfVIP

Repulsive Forces Between Looping Chromosomes Induce Entropy-Driven Segregation Manfred Bohn*, Dieter W. Heermann Institute for Theoretical Physics, Heidelberg University, Heidelberg, Germany Abstract One striking feature of chromatin organization is that chromosomes are compartmentalized into distinct territories during interphase, the degree of intermingling being much smaller than expected for linear chains. A growing body of evidence indicates that the formation of loops plays a dominant role in transcriptional regulation as well as the entropic organization of interphase chromosomes. Using a recently proposed model, we quantitatively determine the entropic forces between chromosomes. This Dynamic Loop Model assumes that loops form solely on the basis of diffusional motion without invoking other long-range interactions. We find that introducing loops into the structure of chromatin results in a multi-fold higher repulsion between chromosomes compared to linear chains. Strong effects are observed for the tendency of a non- random alignment; the overlap volume between chromosomes decays fast with increasing loop number. Our results suggest that the formation of chromatin loops imposes both compartmentalization as well as order on the system without requiring additional energy-consuming processes. Citation: Bohn M, Heermann DW (2011) Repulsive Forces Between Looping Chromosomes Induce Entropy-Driven Segregation. PLoS ONE 6(1): e14428. doi:10.1371/journal.pone.0014428 Editor: Giuseppe Chirico, University of Milano-Bicocca, Italy Received April 22, 2010; Accepted November 16, 2010; Published January 4, 2011 Copyright: 2011 Bohn, Heermann. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided t