rho signaling participates in membrane fluidity homeostasisρ信号参与膜流动性体内平衡.pdfVIP

Rho Signaling Participates in Membrane Fluidity Homeostasis 1,2 3 1¤ 1 1 Daniel Lockshon , Carissa Perez Olsen , Christopher L. Brett , Andrei Chertov , Alexey J. Merz , 4 3 1,2 Daniel A. Lorenz , Marc R. Van Gilst , Brian K. Kennedy * 1 Department of Biochemistry, University of Washington, Seattle, Washington, United States of America, 2 Buck Institute for Age Research, Novato, California, United States of America, 3 Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America, 4 Sonoma State University, Rohnert Park, California, United States of America Abstract Preservation of both the integrity and fluidity of biological membranes is a critical cellular homeostatic function. Signaling pathways that govern lipid bilayer fluidity have long been known in bacteria, yet no such pathways have been identified in eukaryotes. Here we identify mutants of the yeast Saccharomyces cerevisiae whose growth is differentially influenced by its two principal unsaturated fatty acids, oleic and palmitoleic acid. Strains deficient in the core components of the cell wall integrity (CWI) pathway, a MAP kinase pathway dependent on both Pkc1 (yeast’s sole protein kinase C) and Rho1 (the yeast RhoA-like small GTPase), were among those inhibited by palmitoleate yet stimulated by oleate. A single GEF (Tus1) and a single GAP (Sac7) of Rho1 were also identified, neither of which participate in the CWI pathway. In contrast, key components of the CWI pathway, such as Rom2, Bem2 and Rlm1, failed to influence fatty acid sensitivity. The differential influence of palmitoleate and oleate on g