role of meprins to protect ileal mucosa of crohns disease patients from colonization by adherent-invasive e. coli角色meprins保护回肠黏膜的克罗恩病病人从殖民adherent-invasive大肠杆菌.pdfVIP

Role of Meprins to Protect Ileal Mucosa of Crohn’s Disease Patients from Colonization by Adherent-Invasive E. coli 1,2 ` 1,2. ´ 1,2. 3 Emilie Vazeille , Marie-Agnes Bringer , Aurelie Gardarin , Christophe Chambon , Christoph 4 5 6 ¨ 6 7 Becker-Pauly , Sylvia L. F. Pender , Christine Jakob , Stefan Muller , Daniel Lottaz , Arlette Darfeuille-Michaud1,2* ´ ´ 1 Clermont Universite, JE2526 Universite d’Auvergne, Clermont-Ferrand, France, 2 INRA USC 2018, Clermont-Ferrand, France, 3 INRA, Plate-Forme d’Exploration du ´ ` Metabolisme, Saint-Genes-Champanelle, France, 4 Institute of Zoology, Cell and Matrix Biology, Johannes Gutenberg University, Mainz, Germany, 5 Division of Infection, Inflammation, and Immunity, School of Medicine, University of Southampton, Southampton, United Kingdom, 6 Division of Gastroenterology, Department of Clinical Research, University of Bern, Bern, Switzerland, 7 Department of Rheumatology, Clinical Immunology and Allergology, Inselspital, University of Bern, Bern, Switzerland Abstract Ileal lesions in Crohn’s disease (CD) patients are colonized by pathogenic adherent-invasive Escherichia coli (AIEC) able to adhere to and invade intestinal epithelial cells (IEC), and to survive within macrophages. The interaction of AIEC with IEC depends on bacterial factors mainly type 1 pili, flagella, and outer membrane proteins. In humans, proteases can act as host defence mechanisms to counteract bacterial colonization. The protease meprin, composed of multimeric complexes of the two subunit