scavenger receptor mediates systemic rna interference in ticks清道夫受体介导系统性rna干扰在蜱虫.pdfVIP

Scavenger Receptor Mediates Systemic RNA Interference in Ticks 1,2 2 2 2 3 Kyaw Min Aung , Damdinsuren Boldbaatar , Rika Umemiya-Shirafuji , Min Liao , Xuan Xuenan , Hiroshi Suzuki3, Remil Linggatong Galay1,2, Tetsuya Tanaka1,2, Kozo Fujisaki1,2* 1 Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi, Japan, 2 Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima, Japan, 3 National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan Abstract RNA interference is an efficient method to silence gene and protein expressions. Here, the class B scavenger receptor CD36 (SRB) mediated the uptake of exogenous dsRNAs in the induction of the RNAi responses in ticks. Unfed female Haemaphysalis longicornis ticks were injected with a single or a combination of H. longicornis SRB (HlSRB) dsRNA, vitellogenin-1 (HlVg-1) dsRNA, and vitellogenin receptor (HlVgR) dsRNA. We found that specific and systemic silencing of the HlSRB, HlVg-1, and HlVgR genes was achieved in ticks injected with a single dsRNA of HlSRB, HlVg-1, and HlVgR. In ticks injected first with HlVg-1 or HlVgR dsRNA followed 96 hours later with HlSRB dsRNA (HlVg-1/HlSRB or HlVgR/HlSRB), gene silencing of HlSRB was achieved in addition to first knockdown in HlVg-1 or HlVgR, and prominent phenotypic changes were observed in engorgement, mortality, and hatchability, indicating that a systemic and specific double knockdown of target genes