浓香型白酒窖池细菌群落.pdfVIP

微生物学通报 NOV 20, 2010, 37(11): 1621−1627 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 浓香型白酒窖池细菌群落 * 罗惠波 甄攀 黄治国 ( 643000) 摘 要: 利用 PCR-SSCP 技术研究了不同窖龄浓香型白酒窖池细菌群落的变化规律, 结果发现: (1) 所有窖泥样品均出现 9−22 条较清晰的条带, 其中均表现出较高优势度的条带 7 条, 但各样品 优势条带的变化规律不同; (2) 所有窖泥样品平均生物多样性指数都在 1.93−2.82 之间, 随着窖龄 的增加, 相同位置样品的多样性指数总体上呈递增趋势; (3) 同一窖池不同位置样品的PCR-SSCP 图谱相似性指数较高, 在 0.63−1.00 之间, 不同窖龄窖池的 SSCP 图谱相似性指数较低, 在 0.34−0.76 之间。 关键词: 白酒窖池, 细菌, PCR-SSCP, 16S rDNA, 微生物群落 Research on Bacterial Community in Luzhou-flavor Liquors Pit * LUO Hui-Bo ZHEN Pan HUANG Zhi-Guo (Liquor-making Biotechnology Application Key Laboratory of Sichuan Province , Sichuan University of Science Engineering, Zigong, Sichuan 643000, China) Abstract: The changing laws of bacteria community in different pit ages of luzhou-flavor liquor pit were analyzed using PCR-SSCP technique. The results were as follows: bands from No. 9 to No. 22 were showed clearly in all pit mud samples, which included 7 bands with higher dominant degree. But the changing laws of dominant degree among bands were different. Besides, the mean diversity index of all samples kept between 1.93 and 2.82. With the increasing pit age, the diversity index of samples from the same spots generally showed increa