extraction of betulin, trimyristin, eugenol and carnosic acid using water-organic solvent mixtures桦木醇的提取、含量;丁香酚和鼠尾草酸使用water-organic溶剂混合物.pdfVIP

Molecules 2012, 17, 9274-9282; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Extraction of Betulin, Trimyristin, Eugenol and Carnosic Acid Using Water-Organic Solvent Mixtures Fulgentius N. Lugemwa Department of Chemistry, Pennsylvania State University-York, 1031 Edgecomb Avenue, York 17403, PA, USA; E-Mail: ful4@; Tel.: +1-717-771-8409; Fax: +1-717-771-4062 Received: 12 June 2012; in revised form: 26 July 2012 / Accepted: 27 July 2012 / Published: 3 August 2012 Abstract: A solvent system consisting of ethyl acetate, ethyl alcohol and water, in the volume ratio of 4.5:4.5:1, was developed and used to extract, at room temperature, betulin from white birch bark and antioxidants from spices (rosemary, thyme, sage, and oregano) and white oak chips. In addition, under reflux conditions, trimyristin was extracted from nutmeg using the same solvent system, and eugenol from olives was extracted using a mixture of salt water and ethyl acetate. The protocol demonstrates the use of water in organic solvents to extract natural products from plants. Measurement of the free-radical scavenging activity using by ,-diphenyl-1-picrylhydrazyl (DPPH) indicated that the extraction of plant material using ethyl acetate, ethyl alcohol and water (4.5:4.5:1, v/v/v) was exhaustive when carried out at room temperature for 96 h. Keywords: toxic chemicals; liquid extraction; spices; antioxidants 1.