原创力文档single-cell chemical lysis on microfluidic chips with arrays of microwells单细胞microwells的化学溶解的微流控芯片与数组.pdfVIP
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single-cell chemical lysis on microfluidic chips with arrays of microwells单细胞microwells的化学溶解的微流控芯片与数组
Sensors 2012, 12, 347-358; doi:10.3390/s120100347
OPEN ACCESS
sensors
ISSN 1424-8220
/journal/sensors
Article
Single-Cell Chemical Lysis on Microfluidic Chips with
Arrays of Microwells
Chun-Ping Jen 1,*, Ju-Hsiu Hsiao 1 and Nikolay A. Maslov 2
1 Department of Mechanical Engineering and Advanced Institute of Manufacturing with High-Tech
Innovation, National Chung Cheng University, Chia Yi 62102, Taiwan;
E-Mail: ggina03@.tw
2 Khristianovich Institute of Theoretical and Applied Mechanics, Siberian Division,
Russian Academy of Science, Novosibirsk 630090, Russia; E-Mail: nmaslov@itam.nsc.ru
* Author to whom correspondence should be addressed; E-Mail: imecpj@.tw;
Tel.: +886-5-272-0411 ext. 33322; Fax: +886-5-272-0589.
Received: 29 November 2011; in revised form: 26 December 2011 / Accepted: 29 December 2011 /
Published: 30 December 2011
Abstract: Many conventional biochemical assays are performed using populations of
cells to determine their quantitative biomolecular profiles. However, population averages
do not reflect actual physiological processes in individual cells, which occur either on short
time scales or nonsynchronously. Therefore, accurate analysis at the single-cell level has
become a highly attractive tool for investigating cellular content. Microfluidic chips with
arrays of microwells were developed for single-cell chemical lysis in the present study.
The cellular occupancy in 30-μm-diameter microwells (91.45%) was higher than that
in 20-μ
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