small protein-mediated quorum sensing in a gram-negative bacterium小protein-mediated革兰氏阴性细菌群体感应.pdfVIP

Small Protein-Mediated Quorum Sensing in a Gram- Negative Bacterium 1. 1.¤ 1,2. 1 1 Sang-Wook Han , Malinee Sriariyanun , Sang-Won Lee , Manoj Sharma , Ofir Bahar , Zachary Bower1, Pamela C. Ronald1,2* 1 Department of Plant Pathology and the Genome Center, University of California Davis, Davis, California, United States of America, 2 The Department of Plant Molecular System Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin, South Korea Abstract S The rice XA21 pattern recognition receptor binds a type I secreted sulfated peptide, called axY 22, derived from the Ax21 (activator of XA21-mediated immunity) protein. The conservation of Ax21 in all sequenced Xanthomonas spp. and closely related genera suggests that Ax21 serves a key biological function. Here we show that the predicted N-terminal sequence of Ax21 is cleaved prior to secretion outside the cell and that mature Ax21 serves as a quorum sensing (QS) factor in Xanthomonas oryzae pv. oryzae. Ax21-mediated QS controls motility, biofilm formation and virulence. We provide genetic evidence that the Xoo RaxH histidine kinase serves as the bacterial receptor for Ax21. This work establishes a critical role for small protein-mediated QS in a Gram-negative bacterium. Citation: Han S-W, Sriariyanun M, Lee S-W, Sharma M, Bahar O, et al. (2011) Small Protein-Mediated Quorum Sensing in a Gram-Negative Bacterium. PLoS ONE 6(12): e29192. doi:10.1371/journal.pone.0029192 Editor: Dipshikha Chakravortty, Indian Institute of Science, India Received October 28, 2011; Accepted November 22, 2011; Pu