soft fruit traceability in food matrices using real-time pcr软的水果可追溯食品使用实时pcr矩阵.pdfVIP

Nutrients 2009, 1, 316-328; doi:10.3390/nu1020316 OPEN ACCESS nutrients ISSN 2072-6643 /journal/nutrients Article Soft Fruit Traceability in Food Matrices using Real-Time PCR Luisa Palmieri *, Elisa Bozza and Lara Giongo Fondazione Edmund Mach, IASMA Research and Innovation Centre, Genomics and Crop Biology Area, Via E. Mach 1, 38010, San Michele all’Adige (TN), Italy; E-Mails: elisa.bozza@iasma.it (E.B.); lara.giongo@iasma.it (L.G.) * Author to whom correspondence should be addressed: E-Mail: luisa.palmieri@iasma.it. Received: 9 October 2009 / Accepted: 15 December 2009 / Published: 23 December 2009 Abstract: Food product authentication provides a means of monitoring and identifying products for consumer protection and regulatory compliance. There is a scarcity of analytical methods for confirming the identity of fruit pulp in products containing Soft Fruit. In the present work we have developed a very sensible qualitative and quantitative method to determine the presence of berry DNAs in different food matrices. To our knowledge, this is the first study that shows the applicability, to Soft Fruit traceability, of melting curve analysis and multiplexed fluorescent probes, in a Real-Time PCR platform. This methodology aims to protect the consumer from label misrepresentation. Keywords: soft fruit; traceability; real-time PCR; strawberry; blueberry; currant; raspberry; orange; pineapple 1. Introd