soil fertilization leads to a decline in between-samples variability of microbial community δ13c profiles in a grassland fertilization experiment土壤施肥导致微生物群落的样品之间变异性下降δ13c概要文件在一个草地施肥试验.pdfVIP

Soil Fertilization Leads to a Decline in Between-Samples Variability of Microbial Community d13C Profiles in a Grassland Fertilization Experiment 1,4 2 3 1 Stavros D. Veresoglou *, Barry Thornton , George Menexes , Andreas P. Mamolos , Demetrios S. Veresoglou1 1 Laboratory of Ecology and Environmental Protection, Faculty of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece, 2 Environmental and Biochemical Sciences Group, The James Hutton Institute, Craigiebuckler, Aberdeen, United Kingdom, 3 Laboratory of Agronomy, School of Agriculture, Aristotle University of ¨ Thessaloniki, Thessaloniki, Greece, 4 Plant Ecology, Freie Universitat Berlin, Berlin, Germany Abstract Gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) was used to measure the 13C/12C ratios of PLFAs at natural abundance levels from a temperate grassland nitrogen (N) and phosphorus (P) factorial fertilization experiment in northern Greece. In each plot two rhizosphere samples were derived centred around individual Agrostis capillaris and Prunella vulgaris plants. It was hypothesized that the isotopic signal of microbes that preferentially feed on recalcitrant litter such as fungi would be modified by fertilization more strongly than that of opportunistic microbes using labile C. Microbial community d13C was affected by both P and N fertilization regime and plant species identity. However, we have been unable to detect significant nutrient effects on individual groups of microbes when analyzed separately in contrast to our original hypothesis. Intra-treatment variability, as evaluated from Hartley’s Fmax tests in the five first PCA compon