strong nuclear egfr expression in colorectal carcinomas is associated with cyclin-d1 but not with gene egfr amplification强大的核表皮生长因子受体在大肠癌癌与周期蛋白d1的但不是与表皮生长因子受体基因扩增.pdfVIP

Dekanić et al. Diagnostic Pathology 2011, 6:108 /content/6/1/108 RESEARCH Open Access Strong nuclear EGFR expression in colorectal carcinomas is associated with cyclin-D1 but not with gene EGFR amplification 1 2 1 1 3 Andrea Dekanić , Renata Dobrila Dintinjan , Ivana Budisavljević , Sanja Pećanić , Marta Žuvić Butorac and Nives Jonjić1* Abstract Background: Prognostic and predictive significance of epidermal growth factor receptor (EGFR) in colorectal carcinomas (CRCs) is still controversial. The aim of the present study was to explore and correlate membrane and nuclear EGFR and cyclin-D1 protein expression with EGFR gene status of tumor cells. Methods: Immunohistochemical and FISH analysis was performed on 135 archival formalin fixed and paraffin embedded CRCs. Results: Strong membrane and strong nuclear EGFR staining was detected in 16% and 57% of cases, respectively, and strong cyclin-D1 expression in 57% samples. Gene EGFR amplification was identified in 5.9% and polysomy in 7.4% of cases, while 87% showed no EGFR gene changes. A statistically significant difference was only found between tumor grade and expression of membrane EGFR, while nuclear EGFR and cyclin-D1 expression was not associated with the clinicopathologic characteristics analyzed. Tumor cells displaying gene amplification and strong protein membrane EGFR expression overlapped, while EGFR gene status showed no correlation with nuclear EGFR and cyclin-D1. There was no association between membrane EGFR and cyclin-D1, whereas nuclear EGFR expression was strongly related to cyclin-D1 expression. Conclusions: Study results revealed heterogeneity among CRCs, which could have a predictive value by identifying b