substance p is a mechanoresponsive, autocrine regulator of human tenocyte proliferationp物质mechanoresponsive,自分泌人类tenocyte扩散的监管机构.pdfVIP

Substance P Is a Mechanoresponsive, Autocrine Regulator of Human Tenocyte Proliferation 1,2,3 1,3,4 1 3,4 1 Ludvig J. Backman , Gloria Fong , Gustav Andersson , Alexander Scott , Patrik Danielson * ˚ ˚ ˚ 1 Department of Integrative Medical Biology, Anatomy, Umea University, Umea, Sweden, 2 Department of Surgical and Perioperative Sciences, Sports Medicine, Umea ˚ University, Umea, Sweden, 3 Department of Physical Therapy, University of British Columbia, Vancouver, British Columbia, Canada, 4 Centre for Hip Health and Mobility, Vancouver Coastal Health and Research Institute, Vancouver, British Columbia, Canada Abstract It has been hypothesised that substance P (SP) may be produced by primary fibroblastic tendon cells (tenocytes), and that this production, together with the widespread distribution of the neurokinin-1 receptor (NK-1 R) in tendon tissue, could play an important role in the development of tendinopathy, a condition of chronic tendon pain and thickening. The aim of this study was to examine the possibility of endogenous SP production and the expression of NK-1 R by human tenocytes. Because tendinopathy is related to overload, and because the predominant tissue pathology (tendinosis) underlying early tendinopathy is characterized by tenocyte hypercellularity, the production of SP in response to loading/strain and the effects of exogenously administered SP on tenocyte proliferation were also studied. A cell culture model of primary human tendon cells was