supermolecular interaction of ferrocenium with yeast dna and application in electrochemical sensing for hybridization recognition of yeast dna超分子相互作用的ferrocenium酵母dna和电化学传感中的应用酵母基因的杂交识别.pdfVIP

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supermolecular interaction of ferrocenium with yeast dna and application in electrochemical sensing for hybridization recognition of yeast dna超分子相互作用的ferrocenium酵母dna和电化学传感中的应用酵母基因的杂交识别.pdf

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supermolecular interaction of ferrocenium with yeast dna and application in electrochemical sensing for hybridization recognition of yeast dna超分子相互作用的ferrocenium酵母dna和电化学传感中的应用酵母基因的杂交识别

Sensors 2004, 4, 71-83 sensors ISSN 1424-8220 © 2004 by MDPI /sensors Supermolecular Interaction of Ferrocenium with Yeast DNA and Application in Electrochemical Sensing for Hybridization Recognition of Yeast DNA Huangxian Ju*, Baofen Ye and Jiayin Gu Department of Chemistry, State Key Laboratory of Coordination Chemistry, Institute of Chemical Biology, Nanjing University, Nanjing 210093, China, Fax: +86-25-3593593, Tel: +86-25-3593593 * Author to whom correspondence should be addressed. E-mail: hxju@ Received: 30 April 2004 / Accepted: 30 May 2004 / Published: 20 July 2004 Abstract: The supermolecular interactions of single-stranded yeast DNA (ssDNA) and + double-stranded DNA (dsDNA) with ferrocenium (Fc ) both in solution and at the electrode/solution interface were studied with UV spectroscopy and electrochemical method. The interaction of covalently immobilized dsDNA with Fc+ resulted in a change of the electrode behavior from diffusion-controlled to surface-controlled and an increase in the current response of Fc+ reduction, which has been used to develop a novel electrochemical yeast DNA sensor for hybridization recognition of immobilized yeast ssDNA to its complementary ssDNA (cDNA). The adsorption constants of Fc+ on ssDNA and dsDNA