suppression of tumor growth and angiogenesis by a specific antagonist of the cell-surface expressed nucleolin抑制肿瘤生长和血管生成特定的细胞表面表达nucleolin的拮抗剂.pdfVIP

Suppression of Tumor Growth and Angiogenesis by a Specific Antagonist of the Cell-Surface Expressed Nucleolin 1. 2. 1 2 1 Damien Destouches , Diala El Khoury , Yamina Hamma-Kourbali , Bernard Krust , Patricia Albanese , 3 4 4 ´ 1 2 Panagiotis Katsoris , Gilles Guichard , Jean Paul Briand , Jose Courty , Ara G. Hovanessian * ´ ´ ´ 1 CNRS UMR 7149, Universite Paris-Est, Creteil, France, 2 CNRS UPR 2228, Universite Paris Descartes, Paris, France, 3 Laboratory of Molecular Pharmacology, University of ´ Patras, Patras, Greece, 4 CNRS UPR 9021, Institut de Biologie Moleculaire et Cellulaire, Strasbourg, France Abstract Background: Emerging evidences suggest that nucleolin expressed on the cell surface is implicated in growth of tumor cells and angiogenesis. Nucleolin is one of the major proteins of the nucleolus, but it is also expressed on the cell surface where is serves as a binding protein for variety of ligands implicated in cell proliferation, differentiation, adhesion, mitogenesis and angiogenesis. Methodology/Principal Findings: By using a specific antagonist that binds the C-terminal tail of nucleolin, the HB-19 pseudopeptide, here we show that the growth of tumor cells and angiogenesis are suppressed in various in vitro and in vivo experimental models. HB-19 inhibited colony formation in soft agar of tumor cell lines, impaired migration of endothelial cells and formation of capillary-like structur