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synthesis and theoretical study of molecularly imprinted nanospheres for recognition of tocopherols合成和分子印迹识别的团簇的理论研究天然维生素e.pdf

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synthesis and theoretical study of molecularly imprinted nanospheres for recognition of tocopherols合成和分子印迹识别的团簇的理论研究天然维生素e

Molecules 2009, 14, 2985-3002; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Synthesis and Theoretical Study of Molecularly Imprinted Nanospheres for Recognition of Tocopherols Theeraphon Piacham, Chanin Nantasenamat, Thummaruk Suksrichavalit, Charoenchai Puttipanyalears, Tippawan Pissawong, Supanee Maneewas, Chartchalerm Isarankura-Na-Ayudhya * and Virapong Prachayasittikul * Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand * Authors to whom correspondence should be addressed; E-mails: mtcis@mahidol.ac.th (C.I.), mtvpr@mahidol.ac.th (V.P.); Tel.: +66-2-441-4376; Fax: +66-2-441-4380 Received: 13 June 2009; in revised form: 5 August 2009 / Accepted: 11 August 2009 / Published: 12 August 2009 Abstract: Molecular imprinting is a technology that facilitates the production of artificial receptors toward compounds of interest. The molecularly imprinted polymers act as artificial antibodies, artificial receptors, or artificial enzymes with the added benefit over their biological counterparts of being highly durable. In this study, we prepared molecularly imprinted polymers for the purpose of binding specifically to tocopherol (vitamin E) and its derivative, tocopherol acetate. Binding of the imprinted polymers to the template was found to be two times greater than that of the control, non-imprinted polymers, when using only 10 mg of polymers. Optimization of the rebinding solvent ind