systemic effects of ingested lactobacillus rhamnosus inhibition of mast cell membrane potassium (ikca) current and degranulation系统性的影响消化乳杆菌抑制肥大细胞膜钾(ikca)当前和脱粒.pdfVIP

Systemic Effects of Ingested Lactobacillus Rhamnosus: Inhibition of Mast Cell Membrane Potassium (IKCa) Current and Degranulation 1,2 1,4 1 1,3 Paul Forsythe *, Binxiang Wang , Ibrahim Khambati , Wolfgang A. Kunze 1 Brain-Body Institute, McMaster University, Hamilton, Ontario, Canada, 2 Department of Medicine, McMaster University, Hamilton, Ontario, Canada, 3 Department of Psychiatry, McMaster University, Hamilton, Ontario, Canada, 4 Centre for Simulation-Based Learning, McMaster University, Hamilton, Ontario, Canada Abstract Exposure of the intestine to certain strains lactobacillus can have systemic immune effects that include the attenuation of allergic responses. Despite the central role of mast cells in allergic disease little is known about the effect of lactobacilli on the function of these cells. To address this we assessed changes in rat mast cell activation following oral treatment with a strain of Lactobacillus known to attenuate allergic responses in animal models. Sprague Dawley rats were fed with L.rhamnosus JB-1 (1 6109) or vehicle control for 9 days. Mediator release from peritoneal mast cells (RPMC) was determined in response to a range of stimuli. Passive cutaneous anaphylaxis (PCA) was used to assess mast cell responses in vivo. The Ca2+ activated K+ channel (KCa3.1) current, identified as critical to mast cell degranulation, was monitored by whole cell patch-clamp. L.rhamnosus JB-1 treatment lead to significant inhibition of mast cell mediator release in response to a range of stimuli including IgE mediated activation. Furthermore, the PCA response was significantly reduced in treated rats. Patch- clamp studies revealed that RPMC from treated animals were much less responsive to the K