taxcollector modifying current 16s rrna databases for the rapid classification at six taxonomic levels收税人修改当前16 s rrna六点数据库的快速分类分类的水平.pdfVIP

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taxcollector modifying current 16s rrna databases for the rapid classification at six taxonomic levels收税人修改当前16 s rrna六点数据库的快速分类分类的水平.pdf

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taxcollector modifying current 16s rrna databases for the rapid classification at six taxonomic levels收税人修改当前16 s rrna六点数据库的快速分类分类的水平

Diversity 2010, 2, 1015-1025; doi:10.3390/d2071015 OPEN ACCESS diversity ISSN 1424-2818 /journal/diversity Article TaxCollector: Modifying Current 16S rRNA Databases for the Rapid Classification at Six Taxonomic Levels Adriana Giongo, Austin G. Davis-Richardson, David B. Crabb and Eric W. Triplett * Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, 1052 Museum Road, Gainesville, FL 32611-0700, USA; E-Mails: giongo@ (A.G.); adavisr@ (A.G.D.-R.); dcrabb@ (D.B.C.) * Author to whom correspondence should be addressed; E-Mail: ewt@; Tel.: +1-352-392-5430; Fax: +1-352-846-0950. Received: 16 June 2010; in revised form: 14 July 2010 / Accepted: 19 July 2010 / Published: 21 July 2010 Abstract: The high level of conservation of 16S ribosomal RNA gene (16S rRNA) in all Prokaryotes makes this gene an ideal tool for the rapid identification and classification of these microorganisms. Databases such as the Ribosomal Database Project II (RDP-II) and the Greengenes Project offer access to sets of ribosomal RNA sequence databases useful in identification of microbes in a culture-independent analysis of microbial communities. However, these databases do not contain all of the taxonomic levels attached to the published names of the bacterial and archaeal sequences. TaxCollector is a set of scripts developed in Python language that attaches taxonomic information to all 16S rRNA sequences in the RDP-II and Greengenes databases.