the ap-1 binding sites located in the pol gene intragenic regulatory region of hiv-1 are important for viral replicationap-1结合位点位于波尔基因基因内调节地区hiv - 1病毒复制是很重要的.pdfVIP

The AP-1 Binding Sites Located in the pol Gene Intragenic Regulatory Region of HIV-1 Are Important for Viral Replication 1 1¤a ´ 1¤b ˆ 1 Laurence Colin , Nathalie Vandenhoudt , Stephane de Walque , Benoıt Van Driessche , Anna 2 ´ 1 3 1 1 2 Bergamaschi , Valerie Martinelli , Thomas Cherrier , Caroline Vanhulle , Allan Guiguen , Annie David , ` 1, Georges Herbein4, Gianfranco Pancino2, Olivier Rohr3*., Carine Van Lint 1*. Arsene Burny ´ ´ ´ ´ 1 Laboratoire de Virologie Moleculaire, Institut de Biologie et de Medecine Moleculaires (IBMM), Universite Libre de Bruxelles (ULB), Gosselies, Belgium, 2 Institut Pasteur, ´ ´ ´ Unite de Regulation des Infections Retrovirales, Paris, France, 3 IUT Louis Pasteur de Schiltigheim, University of Strasbourg, Schiltigheim, France, 4 Department of Virology, ˆ EA3186, IFR133, Franche-Comte University, Hopital Saint-Jacques, Besanc¸on, France Abstract Our laboratory has previously identified an important intragenic region in the human immunodeficiency virus type 1 (HIV-1) genome, whose complete functional unit is composed of the 5103 fragment, the DNaseI-hypersensitive site HS7 and the 5105 fragment. These fragments (5103 and 5105) both exhibit a phorbol 12-myristate 13-acetate (PMA)-inducible enhancer activity on the herpes si