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the application of next generation sequencing in dna methylation analysis新一代测序的应用dna甲基化分析
Genes 2010, 1, 85-101; doi:10.3390/genes 1010085
OPEN ACCESS
genes
ISSN 2073-4425
/journal/genes
Review
The Application of Next Generation Sequencing in DNA
Methylation Analysis
Yingying Zhang and Albert Jeltsch *
School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, D-28759 Bremen,
Germany; E-Mail: y.zhang@jacobs-university.de
* Author to whom correspondence should be addressed; E-Mail: a.jeltsch@jacobs-university.de;
Tel.: +49 421 200 3247; Fax: +49 421 200 324.
Received: 28 April 2010; in revised form: 1 June 2010 / Accepted: 3 June 2010 /
Published: 4 June 2010
Abstract: DNA methylation is a major form of epigenetic modification and plays essential
roles in physiology and disease processes. In the human genome, about 80% of cytosines in
the 56 million CpG sites are methylated to 5-methylcytosines. The methylation pattern of
DNA is highly variable among cells types and developmental stages and influenced by
disease processes and genetic factors, which brings considerable theoretical and
technological challenges for its comprehensive mapping. Recently various high-throughput
approaches based on bisulfite conversion combined with next generation sequencing have
been developed and applied for the genome wide analysis of DNA methylation. These
methods provide single base pair resolution, quantitative DNA methylation data with
genome wide coverage. We review these methods here and discuss some technical points
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