the cysteine rich necrotrophic effector sntox1 produced by stagonospora nodorum triggers susceptibility of wheat lines harboring snn1半胱氨酸丰富necrotrophic效应产生的sntox1 stagonospora nodorum触发小麦行窝藏snn1的易感性.pdfVIP

The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1 1 1 2 3 3 4 Zhaohui Liu , Zengcui Zhang , Justin D. Faris , Richard P. Oliver , Robert Syme , Megan C. McDonald , 4 5 2 6 2 Bruce A. McDonald , Peter S. Solomon , Shunwen Lu , Weilin L. Shelver , Steven Xu , Timothy L. Friesen1,2* 1 Department of Plant Pathology, North Dakota State University, Fargo, North Dakota, United States of America, 2 Northern Crop Science Laboratory, USDA-ARS, Fargo, North Dakota, United States of America, 3 Department of Environment Agriculture, Curtin University, Perth, Australia, 4 Plant Pathology Group, Institute of Integrative Biology, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland, 5 Research School of Biology, The Australian National University, Canberra, Australia, 6 Biosciences Research Laboratory, USDA-ARS, Fargo, North Dakota, United States of America Abstract The wheat pathogen Stagonospora nodorum produces multiple necrotrophic effectors (also called host-selective toxins) that promote disease by interacting with corresponding host sensitivity gene products. SnTox1 was the first necrotrophic effector identified in S. nodorum, and was shown to induce necrosis on wheat lines carrying Snn1. Here, we report the molecular cloning and validation of SnTox1 as well as the preliminary characterization of the mechanism underlying the SnTox1-Snn1 interaction which leads to susceptibility. SnTox1 was identified using bioinformatics tools and verified by heterologous expression in Pichia pastoris. SnTox1 enc