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Sensors 2009, 9, 8709-8721; doi:10.3390/ OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach Joanne H. Wang 1, Kevin Wang 2, Brandon Bartling 3 and Chung-Chiun Liu 3,* 1 Department of Biology, Brown University, 171 Meeting Street, Providence, RI 02912, USA; E-Mail: jowang89@ (J.H.W.) 2 College of Medicine, University of Cincinnati, 231 Albert B. Sabin Way, Cincinnati, OH 45267, USA; E-Mail: wangkv@ (K.W.) 3 Department of Chemical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA; E-Mail: bxb79@ (B.B.) * Author to whom correspondence should be addressed; E-Mail: cxl9@; Tel.: +1-216-368-2935; Fax: +1-216-368-8738. Received: 22 September 2009; in revised form: 15 October 2009 / Accepted: 16 October 2009 / Published: 30 October 2009 Abstract: A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0–300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exis