the effects of g-csf on proliferation of mouse myocardial microvascular endothelial cells鼠标的g - csf对扩散的影响心肌微血管内皮细胞.pdfVIP

Int. J. Mol. Sci. 2011, 12, 1306-1315; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells Jiming Li 1, Yunzeng Zou 2,3,*, Junbo Ge 2,3,*, Daifu Zhang 1, Aili Guan 2,3, Jian Wu 2,3 and Lei Li 2,3 1 Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 150 Jimo Road, Shanghai 200120, China; E-Mails: lijmsh912@ (J.L.); dfzhangsh912@ (D.Z.) 2 Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200032, China; E-Mails: alguansh912@ (A.G.); jwush912@ (J.W.); llish912@ (L.L.) 3 Institutes of Biomedical Scienses, Fudan University, Shanghai 200032, China * Authors to whom correspondence should be addressed; E-Mails: yzzoush912@ (Y.Z.); jbgesh912@yaho (J.G.); Tel.: +86-21 Fax: +86-2 1 Received: 31 December 2010; in revised form: 7 February 2011 / Accepted: 18 February 2011 / Published: 22 February 2011 Abstract: This paper explores the effect of granulocyte colony-stimulating factor (G-CSF) on mouse myocardial microvascular endothelial cell (CMECs) proliferation. CMECs were harvested from C57/BL6 mice. CMECs were cultured in medium containing G-CSF (0 ng/mL, 20 ng/mL, 40 ng/mL, 60 ng/mL) for five days. Proliferative activity of CMECs was examined by CCK-8 method. Hypoxi