the genetics of symbiotic nitrogen fixation comparative genomics of 14 rhizobia strains by resolution of protein clusters的基因比较基因组学14根瘤菌共生固氮菌株蛋白质集群的决议.pdfVIP

Genes 2012, 3, 138-166; doi:10.3390/genes3010138 OPEN ACCESS genes ISSN 2073-4425 /journal/genes Article The Genetics of Symbiotic Nitrogen Fixation: Comparative Genomics of 14 Rhizobia Strains by Resolution of Protein Clusters Michael Black 1,*, Paula Moolhuijzen 1, Brett Chapman 1, Roberto Barrero 1, John Howieson 2, Mariangela Hungria 3 and Matthew Bellgard 1,* 1 Centre for Comparative Genomics, Murdoch University, South Street, Murdoch, Perth, WA 6150, Australia; E-Mails: pmoolhuijzen@.au (P.M.); bchapman@.au (B.C.); rbarrero@.au (R.B.); 2 Centre for Rhizobium Studies, Murdoch University, South Street, Murdoch, Perth, WA 6150, Australia; E-Mail: j.howieson@.au 3 Embrapa Soja, Cx. Postal 231, Londrina, Parana, 86001-970, Brazil; E-Mail: hungria@cnpso.embrapa.br * Author to whom correspondence should be addressed; E-Mails: mblack@.au; mbellgard@.au. Received: 24 January 2012; in revised form: 10 February 2012 / Accepted: 13 February 2012 / Published: 16 February 2012 Abstract: The symbiotic relationship between legumes and nitrogen fixing bacteria is critical for agriculture, as it may have profound impacts on lowering costs for farmers, on land sustainability, on soil quality, and on mitigation of greenhouse gas emissions. However, despite the importance of the symbioses to the global nitrogen cycling balance, very few rhizobial genomes have been sequenced so far, although there are some ongoing efforts in sequencing elite strains. In this study, the genomes of fourteen selected strains of th