the genome sequence of polymorphum gilvum sl003b-26a1t reveals its genetic basis for crude oil degradation and adaptation to the saline soil的基因组序列polymorphum gilvum sl003b-26a1t揭示其遗传基础原油降解和适应盐渍土.pdfVIP

The Genome Sequence of Polymorphum gilvum SL003B- 26A1T Reveals Its Genetic Basis for Crude Oil Degradation and Adaptation to the Saline Soil Yong Nie, Yue-Qin Tang, Yan Li, Chang-Qiao Chi, Man Cai, Xiao-Lei Wu* Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing Abstract Polymorphum gilvum SL003B-26A1T is the type strain of a novel species in the recently published novel genus Polymorphum isolated from saline soil contaminated with crude oil. It is capable of using crude oil as the sole carbon and energy source and can adapt to saline soil at a temperature of 45uC. The Polymorphum gilvum genome provides a genetic basis for understanding how the strain could degrade crude oil and adapt to a saline environment. Genome analysis revealed the versatility of the strain for emulsifying crude oil, metabolizing aromatic compounds (a characteristic specific to the Polymorphum gilvum genome in comparison with other known genomes of oil-degrading bacteria), as well as possibly metabolizing n-alkanes through the LadA pathway. In addition, COG analysis revealed Polymorphum gilvum SL003B-26A1T has significantly higher abundances of the proteins responsible for cell motility, lipid transport and metabolism, and secondary metabolite biosynthesis, transport and catabolism than the average levels found in all other genomes sequenced thus far, but lower abundances of the proteins responsible for carbohydrate transport and metabolism, defense mechanisms, and translation than the average levels. These traits support the adaptability of Polymorphum gilvum to a crude oil-contaminated saline environment. The Polymorphum gilvum genome could serve as a platform for further study of oil-degrading microorganisms for