the human gonadotropin releasing hormone type i receptor is a functional intracellular gpcr expressed on the nuclear membrane人类的促性腺激素释放激素受体i型是一种功能性细胞内核膜gpcr表示.pdfVIP

The Human Gonadotropin Releasing Hormone Type I Receptor Is a Functional Intracellular GPCR Expressed on the Nuclear Membrane 1,2,3,4. 1,2,3. 5. ´ ´ 5 6 Michelle Re , Macarena Pampillo , Martin Savard , Celena Dubuc , Craig A. McArdle , 7 8 5 4 1,2,3,4 Robert P. Millar , P. Michael Conn , Fernand Gobeil, Jr. *, Moshmi Bhattacharya , Andy V. Babwah * 1The Children’s Health Research Institute, London, Canada, 2 Lawson Health Research Institute, London, Canada, 3 Department of Obstetrics and Gynaecology, The University of Western Ontario, London, Canada, 4 Department of Physiology and Pharmacology, The University of Western Ontario, London, Canada, 5 Department of ´ Pharmacology, Universite de Sherbrooke, Sherbrooke, Canada, 6 Laboratories for Integrated Neuroscience and Endocrinology, Department of Clinical Sciences at South Bristol, University of Bristol, Bristol, United Kingdom, 7 MRC Human Reproductive Sciences Unit, The Queen’s Medical Research Institute, Edinburgh, United Kingdom, 8 Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, United States of America Abstract The mammalian type I gonadotropin releasing hormone receptor (GnRH-R) is a structurally unique G protein-coupled receptor (GPCR) that lacks cytoplasmic tail sequences and displays inefficient plasma membrane expression (PME). Compared to its murine counterparts, the primate type I receptor is inefficiently folded and ret