原创力文档universal oligonucleotide microarray for sub-typing of influenza a virus普遍的寡核苷酸微阵列甲型流感病毒的子类型.pdfVIP
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universal oligonucleotide microarray for sub-typing of influenza a virus普遍的寡核苷酸微阵列甲型流感病毒的子类型
Universal Oligonucleotide Microarray for Sub-Typing of
Influenza A Virus
1 1 1 2
Vladimir A. Ryabinin , Elena V. Kostina , Galiya A. Maksakova , Alexander A. Neverov , Konstantin M.
Chumakov2*, Alexander N. Sinyakov1
1 Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia, 2 Center of Biologics Evaluation and
Research, Food and Drug Administration, Rockville, Maryland, United States of America
Abstract
A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes
allowing viruses to be classified by the subtypes of hemagglutinin (H1–H13, H15, H16) and neuraminidase (N1–N9).
Additional sets of probes are used to detect H1N1 swine influenza viruses. Selection of probes was done in two steps.
Initially, amino acid sequences specific to each subtype were identified, and then the most specific and representative
oligonucleotide probes were selected. Overall, between 19 and 24 probes were used to identify each subtype of
hemagglutinin (HA) and neuraminidase (NA). Genotyping included preparation of fluorescently labeled PCR amplicons of
influenza virus cDNA and their hybridization to microarrays of specific oligonucleotide probes. Out of 40 samples tested, 36
unambiguously identified HA and NA subtypes of Influenza A virus.
Citation: Ryabinin VA, Kostina EV, Maksakova GA, Neverov AA, Chumakov KM, et al. (2011) Universal Oligonucleotide Microarray for Sub-Typing of Influenza A
Virus. PLoS ONE 6(4): e17529. doi:10.1371/journal.pone.0017529
Editor: Ralph Tripp, University of Georgia, United States of America
Received December 27,
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