urinary aminopeptidase activities as early and predictive biomarkers of renal dysfunction in cisplatin-treated rats尿氨肽酶的活动早和预测生物标志物cisplatin-treated老鼠的肾脏功能障碍.pdfVIP
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urinary aminopeptidase activities as early and predictive biomarkers of renal dysfunction in cisplatin-treated rats尿氨肽酶的活动早和预测生物标志物cisplatin-treated老鼠的肾脏功能障碍
Urinary Aminopeptidase Activities as Early and
Predictive Biomarkers of Renal Dysfunction in Cisplatin-
Treated Rats
´ 1 ´ 2 ´ 1 3 ´
Andres Quesada , Felix Vargas , Sebastian Montoro-Molina , Francisco O’Valle , Marıa
´ ´ 3 4 1 ´ 1
Dolores Rodrıguez-Martınez , Antonio Osuna , Isabel Prieto , Manuel Ramırez ,
Rosemary Wangensteen1*
´
´ ´ ´ ´
1 Area de Fisiologıa, Departamento de Ciencias de la Salud, Universidad de Jaen, Jaen, Spain, 2 Departamento de Fisiologıa, Facultad de Medicina, Universidad de
´ ´ ´
Granada, Granada, Spain, 3 Departamento de Anatomıa Patologica, Facultad de Medicina, Universidad de Granada, IBIMER Granada, Spain, 4 Servicio de Nefrologıa,
Hospital Virgen de las Nieves, Granada, Spain
Abstract
This study analyzes the fluorimetric determination of alanyl- (Ala), glutamyl- (Glu), leucyl-cystinyl- (Cys) and aspartyl-
aminopeptidase (AspAp) urinary enzymatic activities as early and predictive biomarkers of renal dysfunction in cisplatin-
treated rats. Male Wistar rats (n = 8 each group) received a single subcutaneous injection of either saline or cisplatin 3.5 or
7 mg/kg, and urine samples were taken at 0, 1, 2, 3 and 14 days afte
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