uropathogenic escherichia coli induces serum amyloid a in mice following urinary tract and systemic inoculationuropathogenic大肠杆菌诱导血清淀粉样蛋白在小鼠接种后尿道和系统性.pdfVIP

Uropathogenic Escherichia coli Induces Serum Amyloid A in Mice following Urinary Tract and Systemic Inoculation 1 2 2 3 4 Andreja Erman , Katja Lakota , Katjusa Mrak-Poljsak , Matthew G. Blango , Veronika Krizan-Hergouth , 3 2 1 Matthew A. Mulvey , Snezna Sodin-Semrl *, Peter Veranic 1 Faculty of Medicine, Institute of Cell Biology, University of Ljubljana, Ljubljana, Slovenia, 2 Department of Rheumatology, University Medical Centre-Ljubljana, Ljubljana, Slovenia, 3 Division of Microbiology and Immunology, Department of Pathology, University of Utah, Salt Lake City, Utah, United States of America, 4 Faculty of Medicine, Institute of Microbiology and Immunology, University of Ljubljana, Ljubljana, Slovenia Abstract Serum amyloid A (SAA) is an acute phase protein involved in the homeostasis of inflammatory responses and appears to be a vital host defense component with protective anti-infective properties. SAA expression remains poorly defined in many tissues, including the urinary tract which often faces bacterial challenge. Urinary tract infections (UTIs) are usually caused by strains of uropathogenic Escherichia coli (UPEC) and frequently occur among otherwise healthy individuals, many of whom experience bouts of recurrent and relapsing infections despite the use of antibiotics. To date, whether SAA is present in the infected urothelium and whether or not the induction of SAA can protect the host against UPEC is unclear. Here we show, using mouse models coupled with immunofluorescence microscopy and quantitative RT-PCR, that delivery of UPEC either directly into the urinary tract via catheterization or systemically via intraperitoneal injection triggers the e