utilization of dispersive liquid-liquid microextraction coupled with hplc-uv as a sensitive and efficient method for the extraction and determination of oleanolic acid and ursolic acid in chinese medicinal herbs英文论文.pdfVIP

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utilization of dispersive liquid-liquid microextraction coupled with hplc-uv as a sensitive and efficient method for the extraction and determination of oleanolic acid and ursolic acid in chinese medicinal herbs英文论文.pdf

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utilization of dispersive liquid-liquid microextraction coupled with hplc-uv as a sensitive and efficient method for the extraction and determination of oleanolic acid and ursolic acid in chinese medicinal herbs英文论文

American Journal of Analytical Chemistry , 2012, 3, 675-682 /10.4236/ajac.2012.310089 Published Online October 2012 (http://www.SciRP.org/journal/ajac) Utilization of Dispersive Liquid-Liquid Microextraction Coupled with HPLC-UV as a Sensitive and Efficient Method for the Extraction and Determination of Oleanolic Acid and Ursolic Acid in Chinese Medicinal Herbs * * Yaomei Hao, Xuan Chen, Shuang Hu , Xiaohong Bai , Deshuang Gu School of Pharmacy, Shanxi Medical University, Taiyuan, China * * Email: bxh246@163.com, hurrah100@ Received August 18, 2012; revised September 19, 2012; accepted September 26, 2012 ABSTRACT Isomeric triterpenic acids of oleanolic acid (OA) and ursolic acid (UA) both have very low ultraviolet absorption and always exist in the same plant, so the separation and simultaneous determination of them have been a difficult task. In this study, a sensitive method combining dispersive liquid-liquid microextraction (DLLME) with HPLC-UV was deve- loped for the extraction and determination of OA and UA in traditional Chinese medicinal herbs (CMHs). Variables in- fluencing DLLME such as type and volume of extraction solvent, volume of dispersive solvent, ionic strength, aqueous phase pH, extraction time, centrifugation speed and time, and sample volume were investigated and optimized. Under the optimum conditions, both OA and UA attained favorable extraction efficiencies with enrichment factors 1378 and 933, respectively. The linear dynamic ranges of 0.07 - 30.4 μg·mL–1 for OA and 0.08 - 33.6 μg·mL–1 for UA were ob- tained with square correlation coefficients of 0.9963. The detection limits of OA and UA were both 0