using the tg(nrdegfp)albino zebrafish line to characterize in vivo expression of neurod使用tg(nrdegfp)白化斑马鱼行描述neurod的体内表达.pdfVIP

Using the Tg(nrd:egfp)/albino Zebrafish Line to Characterize In Vivo Expression of neurod 1 2 2 1 Jennifer L. Thomas , Margaret J. Ochocinska , Peter F. Hitchcock , Ryan Thummel * 1 Department of Anatomy and Cell Biology and Department of Ophthalmology, Wayne State University School of Medicine, Detroit, Michigan, United States of America, 2 Department of Ophthalmology and Visual Sciences, University of Michigan Kellogg Eye Center, Ann Arbor, Michigan, United States of America Abstract In this study, we used a newly-created transgenic zebrafish, Tg(nrd:egfp)/albino, to further characterize the expression of neurod in the developing and adult retina and to determine neurod expression during adult photoreceptor regeneration. We also provide observations regarding the expression of neurod in a variety of other tissues. In this line, EGFP is found in cells of the developing and adult retina, pineal gland, cerebellum, olfactory bulbs, midbrain, hindbrain, neural tube, lateral line, inner ear, pancreas, gut, and fin. Using immunohistochemistry and in situ hybridization, we compare the expression of the nrd:egfp transgene to that of endogenous neurod and to known retinal cell types. Consistent with previous data based on in situ hybridizations, we show that during retinal development, the nrd:egfp transgene is not expressed in proliferating retinal neuroepithelium, and is expressed in a subset of retinal neurons. In contrast to previous studies, nrd:egfp is gradually re-expressed in all rod photoreceptors. During photoreceptor regeneration in adult zebrafish, in situ hybridization reveals ¨ that neurod is not expressed in Muller glial-derived neuronal progenitors, but is expressed in photoreceptor progenitors as they m