原创力文档vaccination with brucella abortus recombinant in vivo-induced antigens reduces bacterial load and promotes clearance in a mouse model for infection疫苗接种在vivo-induced流产布鲁氏菌重组抗原减少细菌负荷,促进间隙感染的小鼠模型.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
vaccination with brucella abortus recombinant in vivo-induced antigens reduces bacterial load and promotes clearance in a mouse model for infection疫苗接种在vivo-induced流产布鲁氏菌重组抗原减少细菌负荷,促进间隙感染的小鼠模型
Vaccination with Brucella abortus Recombinant In Vivo-
Induced Antigens Reduces Bacterial Load and Promotes
Clearance in a Mouse Model for Infection
1,3 2 1 1 1 1
Jake E. Lowry *, Dale D. Isaak , Jack A. Leonhardt , Giulia Vernati , Jessie C. Pate , Gerard P. Andrews *
1 Department of Veterinary Sciences, University of Wyoming, Laramie, Wyoming, United States of America, 2 Department of Molecular Biology, University of Wyoming,
Laramie, Wyoming, United States of America, 3 Professional Veterinary Medicine Program, College of Veterinary Medicine Biomedical Sciences, Colorado State
University, Fort Collins, Colorado, United States of America
Abstract
Current vaccines used for the prevention of brucellosis are ineffective in inducing protective immunity in animals that are
chronically infected with Brucella abortus, such as elk. Using a gene discovery approach, in vivo-induced antigen technology
(IVIAT) on B. abortus, we previously identified ten loci that encode products up-regulated during infection in elk and
consequently may play a role in virulence. In our present study, five of the loci (D15, 0187, VirJ, Mdh, AfuA) were selected for
further characterization and compared with three additional antigens with virulence potential (Hia, PrpA, MltA). All eight
genes were PCR-amplified from B. abortus and cloned into E. coli. The recombinant products were then expressed, purified,
adjuvanted, and delivered subcutaneously to BALB/c mice. After primary immunization and two boosts, mice were
4
challenged i.p. with 5 610 CFU of B. abortus strain 19. Spleens from challenged animals were harvested and bacterial loads
determin
您可能关注的文档
- unconventional transcriptional response to environmental enrichment in a mouse model of rett syndrome非传统的转录反应环境浓缩rett综合症的小鼠模型.pdf
- uncoupling protein-4 (ucp4) increases atp supply by interacting with mitochondrial complex ii in neuroblastoma cells解偶联含有(ucp4)增加atp供应与线粒体交互复杂二世在神经母细胞瘤细胞.pdf
- uncovering suitable reference proteins for expression studies in human adipose tissue with relevance to obesity发现合适的参考蛋白表达在人类脂肪组织与肥胖的相关性研究.pdf
- uncertainty determination methodology, sampling maps generation and trend studies with biomass thermogravimetric analysis不确定性测定方法,抽样地图生成与生物质热重量分析和趋势研究.pdf
- uncovering genes with divergent mrna-protein dynamics in streptomyces coelicolor揭示基因与发散mrna-protein动力学链霉菌属coelicolor.pdf
- uncovering the prevalence and diversity of integrating conjugative elements in actinobacteria发现的普遍性和多样性结合共轭元素放线菌.pdf
- uncovering the profile of somatic mtdna mutations in chinese colorectal cancer patients揭示体细胞mtdna突变的形象在中国结直肠癌患者.pdf
- uncovering the molecular machinery of the human spindle—an integration of wet and dry systems biology揭露人类的分子机械spindle-an集成湿和干燥系统生物学.pdf
- uncovering the properties of energy-weighted conformation space networks with a hydrophobic-hydrophilic model发现的性质与hydrophobic-hydrophilic energy-weighted构象空间网络模型.pdf
- underlying factors associated with anemia in amazonian children a population-based, cross-sectional study潜在因素与贫血有关亚马逊的孩子以人群为基础的横断面研究.pdf
- vaccination targeting a surface sialidase of p. acnes implication for new treatment of acne vulgaris疫苗接种目标表面唾液酸酶pu2022曼秀雷敦新治疗寻常痤疮的含义.pdf
- vaccine potential of nipah virus-like particles尼帕病毒样颗粒疫苗的潜力.pdf
- vaccine escape recombinants emerge after pneumococcal vaccination in the united states疫苗逃脱重组出现后,接种肺炎球菌疫苗在美国.pdf
- vaccines against a major cause of abortion in cattle, neospora caninum infection疫苗在牛流产的主要原因,neospora caninum感染.pdf
- vaccine efficacy against malaria by the combination of porcine parvovirus-like particles and vaccinia virus vectors expressing cs of plasmodium对疟疾的疫苗功效的结合猪parvovirus-like粒子和牛痘病毒载体表达cs的疟原虫.pdf
- utilizing the exergy concept to address environmental challenges of electric systems利用电力系统(火用)的概念来解决环境挑战.pdf
- vaccinia virus protein c6 is a virulence factor that binds tbk-1 adaptor proteins and inhibits activation of irf3 and irf7牛痘病毒蛋白质c6毒性因子,结合tbk-1适配器蛋白质和抑制irf3和irf7的激活.pdf
- utilization of a biodegradable mulch sheet produced from poly(lactic acid)ecoflex？modified starch in mandarin orange groves利用生物可降解地膜表由聚(乳酸)ecoflex 改性淀粉在柑桔林.pdf
- vaccinia virus–encoded ribonucleotide reductase subunits are differentially required for replication and pathogenesis牛痘病毒编码核苷酸还原酶亚基不同需要复制和发病机理.pdf
- vaginal microbiome and epithelial gene array in post-menopausal women with moderate to severe dryness在绝经后的妇女阴道微生物和上皮基因阵列中度到重度的干燥.pdf
文档评论（0）