variation of the nuclear, subnuclear and chromosomal flavanol deposition in hemlock and rye亚核及染色体变异的核,黄烷醇沉积在铁杉和黑麦.pdfVIP

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variation of the nuclear, subnuclear and chromosomal flavanol deposition in hemlock and rye亚核及染色体变异的核,黄烷醇沉积在铁杉和黑麦.pdf

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variation of the nuclear, subnuclear and chromosomal flavanol deposition in hemlock and rye亚核及染色体变异的核,黄烷醇沉积在铁杉和黑麦

Int. J. Mol. Sci. 2007, 8, 635-650 International Journal of Molecular Sciences ISSN 1422-0067 © 2007 by MDPI /ijms/ Full Research Paper Variation of the Nuclear, Subnuclear and Chromosomal Flavanol Deposition in Hemlock and Rye Walter Feucht 1, Heike Dithmar 2 and Jürgen Polster 2,* 1 Department for Plant Science, Center of Life Science Weihenstephan (WZW), Technical University Munich (TUM), D-85354 Freising, Germany 2 Physical Biochemistry, Research Department Biosciences, WZW, TU Munich (TUM), D-85354 Freising, Germany; E-mail: hdithmar@wzw.tum.de; E-mail: j.polster@wzw.tum.de * Author to whom correspondence should be addressed; E-mail: j.polster@wzw.tum.de Received: 27 April 2007; in Revised Form: 31 May 2007 / Accepted: 27 June 2007 / Published: 10 July 2007 Abstract: Nuclei of hemlock (Tsuga canadensis and Tsuga canadensis var. nana) were investigated for the presence of flavanols. Histochemical staining with p- dimethylaminocinnamaldehyde proved to be a highly valuable method yielding a bright blue flavanol coloration for nuclei. There was a significant variation in flavanol deposition (1) among nuclei, (2) at the subnuclear level and also (3) along the chromosomes during mitosis. The presence of flavanols in nucleoli could not be established probably because they were too small, measuring less than 1 µm in diameter. In contrast to Tsuga, the cells and nuclei of rootlets