unique proteomic signatures distinguish macrophages and dendritic cells独特的蛋白质组签名识别巨噬细胞和树突细胞.pdfVIP

Unique Proteomic Signatures Distinguish Macrophages and Dendritic Cells 1,4 1,4 2,4 1,4 1,4 3 Lev Becker *, Ning-Chun Liu , Michelle M. Averill , Wei Yuan , Nathalie Pamir , Yufeng Peng , Angela D. Irwin1,4, Xiaoyun Fu1,4, Karin E. Bornfeldt2,4, Jay W. Heinecke1,4 1 Department of Medicine, University of Washington, Seattle, Washington, United States of America, 2 Department of Pathology, University of Washington, Seattle, Washington, United States of America, 3 Department of Rheumatology, University of Washington, Seattle, Washington, United States of America, 4 Diabetes and Obesity Center of Excellence, University of Washington, Seattle, Washington, United States of America Abstract Monocytes differentiate into heterogeneous populations of tissue macrophages and dendritic cells (DCs) that regulate inflammation and immunity. Identifying specific populations of myeloid cells in vivo is problematic, however, because only a limited number of proteins have been used to assign cellular phenotype. Using mass spectrometry and bone marrow- derived cells, we provided a global view of the proteomes of M-CSF-derived macrophages, classically and alternatively activated macrophages, and GM-CSF-derived DCs. Remarkably, the expression levels of half the plasma membrane proteins differed significantly in the various populations of cells derived in vitro. Moreover, the membrane proteomes of macrophages and DCs were more distinct than those of classically and alternatively activated macrophages. Hierarchical cluster and dual statistical analyses demonstrated that each cell type exhibited a robust proteomic sign