vitamin a deficiency induces congenital spinal deformities in rats老鼠体内维生素a缺乏症引起先天性脊柱畸形.pdfVIP

Vitamin A Deficiency Induces Congenital Spinal Deformities in Rats 1 1 2 2 1 1 Zheng Li , Jianxiong Shen *, William Ka Kei Wu , Xiaojuan Wang , Jinqian Liang , Guixing Qiu , Jiaming Liu1 1 Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing, China, 2 Department of Medicine and Therapeutics, Institute of Digestive Diseases, LKS Institute of Health Science, The Chinese University of Hong Kong, Hong Kong, China Abstract Most cases of congenital spinal deformities were sporadic and without strong evidence of heritability. The etiology of congenital spinal deformities is still elusive and assumed to be multi-factorial. The current study seeks to elucidate the effect of maternal vitamin A deficiency and the production of congenital spinal deformities in the offsping. Thirty two female rats were randomized into two groups: control group, which was fed a normal diet; vitamin A deficient group, which were given vitamin A-deficient diet from at least 2 weeks before mating till delivery. Three random neonatal rats from each group were killed the next day of parturition. Female rats were fed an AIN-93G diet sufficient in vitamin A to feed the rest of neonates for two weeks until euthanasia. Serum levels of vitamin A were assessed in the adult and filial rats. Anteroposterior (AP) spine radiographs were obtained at week 2 after delivery to evaluate the presence of the skeletal abnormalities especially of spinal deformities. Liver and vertebral body expression of retinaldehyde dehydrogenase (RALDHs) and RARs mRNA was assessed by reverse transcription-real time PCR. VAD neonates displayed many skeletal malformations in the cervical, thoracic, the pelvic and