wall teichoic acids of staphylococcus aureus limit recognition by the drosophila peptidoglycan recognition protein-sa to promote pathogenicity墙磷壁酸的金黄色葡萄球菌限制识别果蝇肽聚糖识别protein-sa促进致病性.pdfVIP

Wall Teichoic Acids of Staphylococcus aureus Limit Recognition by the Drosophila Peptidoglycan Recognition Protein-SA to Promote Pathogenicity Magda L. Atilano1., James Yates1., Marcus Glittenberg2., Sergio R. Filipe1*, Petros Ligoxygakis2* ´ ´ 1 Laboratory of Bacterial Cell Surfaces and Pathogenesis, Instituto de Tecnologia Quımica e Biologica/Universidade Nova de Lisboa, Oeiras, Portugal, 2 Genes and Development Laboratory, Department of Biochemistry, University of Oxford, Oxford, United Kingdom Abstract The cell wall of Gram-positive bacteria is a complex network of surface proteins, capsular polysaccharides and wall teichoic acids (WTA) covalently linked to Peptidoglycan (PG). The absence of WTA has been associated with a reduced pathogenicity of Staphylococcus aureus (S. aureus). Here, we assessed whether this was due to increased detection of PG, an important target of innate immune receptors. Antibiotic-mediated or genetic inhibition of WTA production in S. aureus led to increased binding of the non-lytic PG Recognition Protein-SA (PGRP-SA), and this was associated with a reduction in host susceptibility to infection. Moreover, PGRP-SD, another innate sensor required to control wild type S. aureus infection, became redundant. Our data imply that by using WTA to limit access of innate immune receptors to PG, under-detected bacteria are able to establish an infection and ultimately overwhelm the host. We propose that different PGRPs work in concert to counter this strategy. Citation: Atilano ML, Yates J, Glittenberg M, Filipe SR, Ligoxygakis P (2011) Wall Teichoic Acids of Staphylococcus aureus Limit Recognition by the Drosophila Peptidoglycan Recognition Protein-SA to Promote Pathogenicity. PLoS Pathog 7