通心络对自发性高血压大鼠心肌纤维化影响.docVIP

通心络对自发性高血压大鼠心肌纤维化影响 　 作者：谭安雄　李小云　王玉银　李涛　封芬 【摘要】 目的探讨通心络(Tongxinluo,TXL)对自发性高血压大鼠(spontaneously hypertensive rat,SHR)心肌纤维化的影响。方法将18 只SHR随机分为通心络治疗组(TXL) 和空白对照组(SHR),每组各9 只,另9 只Wistar-Kyoto大鼠作为正常对照组(WKY)。TXL组用通心络溶于蒸馏水中配成溶液后按1.5 g·kg-1·d -1 的剂量灌胃, SHR组、WKY组均给予等量蒸馏水灌胃。1次/d，连续8周后，用M超测量舒张末期左室内径(left ventricular end-diastolic dimension ,LVEDD) 、舒张末期左室后壁厚度(left ventricular posterior walldepth,LVPWD)、舒张末期室间隔厚度(interventricular septal depth,IVSD)；超声测量室间隔的平均声学强度(average image intensity , AII1)、峰- 峰强度(peak to peak intensity , PPI1)及其心包校正的声学强度( corrected acoustic intensity ,CAI1)；左室后壁的平均声学强度(average image intensity , AII2)、峰- 峰强度(peak to peak intensity , PPI2)及其心包校正的声学强度( corrected acoustic intensity ,CAI2)；测定左室质量指数( left ventricular mass index , LVMI)；用放射免疫法测定血清Ⅲ型前胶原氨基末端肽(procollagen type Ⅲamino-terminal propeptide, PⅢNP)。结果TXL组大鼠LVMI、LVPWD、IVSD、AII1、AII2、CAI1、CAI2、PⅢNP较SHR大鼠减小(Plt;0.05)；TXL组大鼠LVEDD、PPI1、PPI2 较SHR组增大(Plt; 0. 05)。结论通心络可抑制SHR心肌纤维化,改善心脏功能。 【关键词】 通心络；纤维化；大鼠；高血压 　　Abstract：ObjectiveTo explore the effect of Tongxinluo on myocardialfibrosis of rats with spontaneous hypertension Methods18 rats with spontaneous hypertension (SHRs) were divided at random into two groups :Tongxinluo group (n=9) and SHR group (n=9).Another 9 Wistar-Kyoto rats served as control group .Rats in Tongxinluo group were fed with a mixture of Tongxinluo and distilled water with dosage of 1.5 g·kg-1·d -1 , while other two groups were fed with distilled water at equal volume,once a week.8 weeks later, left ventricular end-diastolic dimension (LVEDD) , left ventricular posterior wall depth (LVPWD) and interventricular septal depth (IVSD) of late diastolic time were all determined by ultrasonic M .The average image intensity ( AII),the peak to peak intensity (PPI) and the corrected acoustic intensity (CAI) of the interventricular septum and left ventricular posterior wall were all determined by ultrasonic, left ventricular mass index ( LVMI) was determined ,and procollagen type Ⅲamino-terminal propept