COX-2、VEGF、MMP-2在非小细胞肺癌中表达及其意义.doc

COX-2、VEGF、MMP-2在非小细胞肺癌中表达及其意义 　 作者：翁晓丹，尤庆生，施公胜，黄华 【摘要】 目的：探讨环氧化酶－2（COX-2）表达水平与非小细胞肺癌患者预后、肿瘤细胞浸润、侵袭以及新血管生成间的关系。方法：选取手术切除的肺癌标本72例，应用免疫组化方法研究肿瘤组织中COX-2、基质金属蛋白酶－2（MMP-2）、血管内皮生长因子（VEGF）的表达情况。结果：在非小细胞肺癌中COX-2的阳性表达率为54.17%，其中鳞癌的阳性表达率为43.58%，腺癌的阳性表达率为66.66%，两者间差异有统计学意义(Plt;0.05)；COX-2表达还与肿瘤的组织分级、临床病理分期相关。COX-2在有淋巴结转移组中的表达明显高于无淋巴结转移组，尽管两组表达差异无统计学意义(Pgt;0.05)。COX-2的表达与VEGF、MMP-2表达成正相关。结论： COX-2是非小细胞肺癌术后患者独立的不良预后指标,COX-2可能通过促进肿瘤血管新生和浸润转移，在非小细胞肺癌的发展中扮演重要角色。 【关键词】 非小细胞肺癌；环氧化酶－2；基质金属蛋白酶－2；血管内皮生长因子；免疫组织化学 [Abstract] Objective: To study the expression of COX-2 in non-small cell lung carcinoma(NSCLC) and its relationship with prognosis and to investigate the expression relationship between COX-2，VEGF and MMP-2 to research into the role of COX-2 in NSCLC. Methods: The expression of COX-2、VEGF and MMP-2 was examined with immunohistochemical technique in 72 specimens of NSCLC resected by surgery. Results: The positive expression rate of COX-2 in NSCLC, squamous cell carcinoma and adenocarcinoma were 54.17%, 43.58%, 66.66% respectively. The expressive variation of COX-2 in SCC and AC had signigicant difference(Plt;0.05), There was a statistically significant correlationship between COX-2 expression and histological grade and TNM stage. COX-2 expression was significantly higher in sample with lymph node metastases, although there was no significant correlation between COX-2 and lymph node metastases(Pgt;0.05).In NSCLC,the positive rate of VEGF and MMP-2 were 68% and 65%. Result suggest that COX-2 expression was highly correlated to VEGF and MMP-2 expression. Survival analysis suggested COX-2 expression was an independent predictor of survival.Conclusion: COX-2 may be a useful independent poor prognostic factor of surgically resected NSCLC patients.It?s correlation to VEGF and MMP-2 indicated that COX-2 maybe play a role in NSCLC progression via angiogenesis and accelerating invasion. [Key words] Non-small cell lung cancer; Cyclooxygenase-2; Vascular endothelial growth factor; Matrix