RSV毛细支气管炎RANTES MIP-1α IL-8水平变化及相关性探究.docVIP

RSV毛细支气管炎RANTES MIP-1α IL-8水平变化及相关性探究 　 作者：董琳 陈小芳 周晓聪 林剑 李锦燕 张伟 【摘要】 　　目的 探讨呼吸道合胞病毒（RSV）毛细支气管炎(简称RSV毛支)RANTES、MIP-1α、IL-8的动态变化及相关性。 方法 RSV毛支组26例为住院的RSV毛细支气管炎患儿，年龄1.5～11.0个月；对照组为14例择期手术患儿，年龄5.4～15.1个月。应用ELISA法分别检测RSV毛支组急性期和恢复期鼻咽分泌物RANTES、MIP-1α、IL-8浓度。 结果 对照组鼻咽分泌物未测到RANTES和MIP-1α，测得低水平的IL-8；RSV毛细支气管炎患儿急性期RANTES、MIP-1α、IL-8明显高于对照组；恢复期RANTES、IL-8下降，但仍高于对照组；MIP-1α恢复期和急性期差异无显著性（Pgt;0.05）,但高于对照组（Plt;0.001）。RANTES与MIP-1α、IL-8及MIP-1α与IL-8间有正相关关系。 结论 RSV毛细支气管炎患儿RANTES、MIP-1α、IL-8明显升高，提示趋化因子可能在RSV感染的气道炎症中起重要作用。 【关键词】 趋化因子 鼻咽分泌物 毛细支气管炎 呼吸道合胞病毒 　　【Abstract】Objectives To explore the dynamic change of RANTES. MIP-1α and IL-8 in children with respiratory syncytial virus (RSV) bronchiolitis and their correlation . Methods Twenty-six children aged 1.5～11.0（4.7±2.6）month hospitalized with bronchiolitis were enrolled as patient group. RSV infection diagnosis was based on direct immunofluorescence assay in NPS. Forteen children aged 5.4～15.1 month undergoing surgical operation were served as control subjects. NPS samples were collected from patients at acute stage and recovery stage respectively. All samples were analyzed for RANTES. MIP-1α and IL-8 levels with ELISA. Results RANTES and MIP-1αconcentrations were not detected in NPS samples from control patients. Only low levels of IL-8 were detected. Levels of RANTES.MIP-1α and IL-8 were significantly greater in samples obtained from patients at acute stage than those of control children. Levels of RANTES and IL-8 decreased significantly in patients at recovery stage and were greater than those in controls. Levels of MIP-1α in patients at recovery stage had no difference compared with those at acute stage, but were still higher than those in controls. Moreover, RANTES levels positively correlated both with MIP-1α and IL-8 levels; Positive correlation between MIP-1α and IL-8 levels was also apparent. Conclusion NPS concentrations of RANTES.MIP-1α and IL-8 in children with RSV bronchiolitis increase significantly, which suggests an imp