Ⅱ型胶原海绵材料特性及对软骨缺损影响.docVIP

Ⅱ型胶原海绵材料特性及对软骨缺损影响 　 作者：廖鹰扬，陈鸿辉，杨小红，梁佩红，戴丽冰，谭见容 【摘要】 [目的]研究Ⅱ型胶原海绵的材料特性与其在修复软骨缺损中的作用。[方法]对比Ⅱ型胶原海绵与Ⅱ型胶原膜的力学、孔径方面等材料特性的差异，并建立兔膝关节软骨缺损模型，A组接受Ⅱ型胶原海绵移植，B组接受Ⅱ型胶原膜移植，而C组为空白对照组，术后不予治疗，分别于2、6和12周取材，通过组织学染色观察软骨缺损处修复的效果。[结果]Ⅱ型胶原海绵与Ⅱ型胶原膜在材料特性上有显著差异，胶原自发荧光分层扫描通过图像分析得出A组平均孔径为(93.26±38.41) μm，弹性模量(0.098±0.0 017) MP/m2，均较B组更为理想。组织学显示A组6周时生成大量新生软骨，12周时新生软骨形态与成熟度已经接近正常，较B、C两组更为优越。[结论]Ⅱ型胶原海绵有理想的孔径和合理的生物力学结构性质，有利于软骨缺损的修复，是一种具有良好材料特性的组织工程基质。 【关键词】 组织工程; Ⅱ型胶原; 生物力学; 孔径; 软骨缺损 　　Abstract: [Objective]To study the material characteristics of collagen typeⅡ and its efficacy in repairing cartilage defect. [Methods]Differences in material characteristics such as mechanism,pore-diameter between the two kinds of materials were compared.The cartilage defect model of rabbit knee joint were constructed.In group A,cartilage defects was repaired by spongy-collagen type Ⅱ.In group B,by membrane-collagen type Ⅱ while group C received no treatment.In 2,6,12 weeks postoperatively,the specimens were sacrificed and repair effects were observed with histological staining method.[Results]Significant differences had been found between the two kinds of material. In group A ,the mean diameter was (93.26±38.41)μm and the mean modulus of elasticity was(0.098±0.0017) MP/m2,which were better than those in group B. In group A,6 weeks postoperatively, a great deal of neogenetic cartilage was observed and in 12 weeks postoperatively, the shape and maturity of neogenetic cartilage were observed close to normal level in group A, which had advantages over those in group B、C.[Conclusion]Spongy-collegan type Ⅱ has fairly rational biomechanics performances and proved to have significant efficacy in cartilage defect. To conclude ,it is a kind of tissue-engineering matrix with good material characteristics. 　　Key words：tissue-engineering; collagen type Ⅱ; biomechanics; pore diameter; cartilage defect 　　近年来,大量的文献[1、2]资料显示,胶原产品越来越广泛地被应用于组织工程与相关临床研究。而材料成型工艺、材料特性对修复软骨疗效有着显著影响[3～6]，因此,本文以改进方法提取纯化Ⅱ型胶原，并且以两种不同的成型方法分别制成Ⅱ型胶原膜与Ⅱ型胶原海绵，通过