一肾一夹肾血管性高血压动物模型制作.docVIP

一肾一夹肾血管性高血压动物模型制作 　 作者：孙琪　黄荣桂　郑兴中　梁萌 【摘要】 　　目的 研究一肾一夹（1K1C）肾血管性高血压动物模型的制作。方法 选用200~220 g 体重的SD大鼠，制作1K1C高血压大鼠模型。随机分为正常对照组、单肾对照组(1K)、1K1C组、1K1C+BEA组。使用药物2溴乙胺氢溴酸盐(BEA)破坏肾髓质。采用尾动脉袖套法测量各组大鼠血压的变化。结果 1K组大鼠血压4周后有明显升高，与正常对照组比较，差异有统计学意义（Plt;0.05），但是1K组大鼠与正常对照组大鼠4周后血压远未达到肾血管性高血压入选标准；1K1C组大鼠血压在1周后开始升高，2周时已达到本实验高血压入选标准；1K1C+BEA组血压升高更明显，4周时血压与1K1C组比较，差异有统计学意义（Plt;0.05）。结论 本方法制作肾血管性高血压模型简单易行，成功率约80%。 【关键词】 一肾一夹；高血压；动物模型 　　　Abstract： Objective To study the preparation of onekidney and oneclip (1K1C) renovascular hypertension animal model. Methods The animal models of 1K1C renovascular hypertension were established with SpragueDawley rats (200-220g per rat). The rats were randomly divided into four groups, i.e. normal group, onekidney (1K) group, 1K1C group and 1K1C+BEA group (BEA=2bromoethylamine hydrobromide). The renal medulla was destroyed by BEA. Blood pressure was measured with tailcuff method by rat blood pressure meter of type RBP1. Results Compared with normal control group, the blood pressure in 1K group obviously increased (Plt;0.05), but four weeks later the blood pressure in both the groups did not achieved the standard of renovascular hypertension. The blood pressure in 1K1C group began to increase one week later and achieved the threshold standard of renovascular hypertension two weeks later. Compared with 1K1C group the blood pressure in 1K1C+BEA group increased more obviously after four weeks (Plt;0.05). Conclusion Our making method to rat model of renovascular hypertension is simple and easy to operate. The success rate is about 80%, similar with those in the previous reports. 　　Key words： onekidney one clip; hypertension; animal model 肾血管性高血压(renovascular hypertension)是指单侧或双侧肾动脉的主干或其分支狭窄，使肾血流量减少，导致肾缺血引起的高血压，为继发性高血压最常见的病因。我国肾血管性高血压占高血压人群的5%~7%[1]，肾血管的损害尚可引起受累肾尿的生成及内分泌功能异常。研究方法常以大鼠为实验动物，其动物模型制作有两种类型：二肾一夹(2K1C)型(一侧肾动脉狭窄，保留对侧肾脏)，其高血压早期系“肾素依赖型”；一肾一夹(1K1C)型(一侧肾动脉狭窄，对侧肾切除)，其高血压系“容量依赖型”。钳夹肾动脉的方法有丝线结扎、U型银夹。但丝线结扎对于以后去夹不利，而银夹不易制得，其大小不易控制。本研究参考傅继华等[2]的方法加以改进制作“Ω”形