七总皂甙对低氧高二氧化碳性肺血管收缩影响.docVIP

七总皂甙对低氧高二氧化碳性肺血管收缩影响 　【摘要】 目的:探讨三七总皂甙（PNS）对肺动脉平滑肌的舒张作用及其与肺动脉内皮细胞的关系。方法:采用大鼠离体肺主动脉环灌流模型。分别在常氧及低氧高二氧化碳条件下，观察PNS对氯化钾（KCl）及去甲肾上腺素（NE）预收缩内皮完整血管和去内皮血管的舒张作用。结果:①在常氧及低氧高二氧化碳条件下，累积浓度的PNS对60 mmol/L KCl及10-5 mol/L NE预收缩的血管环均产生剂量依赖性舒张作用(P＜0.05)。②低氧高二氧化碳增强了PNS的肺血管舒张作用，与相应浓度各常氧组相比，均有统计学意义(P＜0.05 或P＜0.01)。③常氧条件下，去内皮组与内皮存在组相比，PNS的血管舒张效应无明显差异(P＞0.05)；但低氧高二氧化碳条件下，去内皮组的PNS血管舒张效应远大于内皮完整组(P＜0.05或P＜0.01)。结论:PNS可减轻低氧高二氧化碳性肺血管收缩，且去除肺动脉内皮后这种作用更加明显，其机制可能与内皮介导作用的缺失有关。 【关键词】 三七总皂甙 低氧 高碳酸血 内皮细胞 Abstract: Objective:To investigate the effect of Panax notogino side(PNS) on the main pulmonary artery rings and the underlying mechanisms involving in the role of endothelial cell. Methods: The model of isolated rat pulmonary artery rings in organ baths was performed. Under normoxia and hypoxia hypercapnia conditions, the effect of accumulated PNS on pulmonary artery rings which were pre-constricted by KC1 or norepinephrine(NE) was observed. And the endothelium was removed in part of these pulmonary artery rings. Result: ①Not only under normoxia conditions but also hypoxia hypercapnia conditions, there was concentration-dependent relaxation in pulmonary artery rings pre-constricted by KC1（60 mmol/L）, with or without endothelium. Similar results occurred in those pulmonary artery rings which were pre-constricted by NE(10-5 mol／L )（P＜0.05）. ②The relaxation effect of PNS on pulmonary artery rings was enhanced under hypoxia hypercapnia conditions. There was statistically significant relaxation in every hypoxia hypercapnia group compared with normoxia group(P＜0.05 or P＜0.01). ③Under normoxia conditions, different vaso-dilation did not occur between endothelium-denuded pulmonary artery rings and endothelium-integrated ones(P＞0.05). But under hypoxia hypercapnia conditions, PNS caused dramatically relaxation in endothelium-denuded pulmonary artery rings rather than endothelium-integrated ones(P＜0.05 or P＜0.01). Conclusion: Under hypoxia hypercapnia conditions, PNS can relax the rat pulmonary rings. Furthermo