冷保存对大鼠部分移植肝再生影响.docVIP

冷保存对大鼠部分移植肝再生影响 　 作者：袁晟光 王继见 董家鸿 【摘要】 目的 探讨冷保存对大鼠部分肝移植术后肝再生的影响。方法 健康SD大鼠分为Ⅰ组(肝切除组)、Ⅱ组(冷保存1 h部分肝移植组)和Ⅲ组(冷保存8 h部分肝移植组)。观察各实验组生存率，比较各组术后1、6、12、24、48、72、168 h肝质量/体质量比率、肝再生率、有丝分裂指数及增殖细胞核抗原表达。结果 Ⅰ、Ⅱ、Ⅲ组7 d存活率分别为100%、90%、40%；Ⅲ组术后2～3 d大鼠肝质量/体质量比率、肝再生率、有丝分裂指数较Ⅰ、Ⅱ组明显偏低(Plt;0.05)；Ⅲ组术后12 h内增殖细胞核抗原表达较其余两组明显偏低(Plt;0.05)，48 h才达高峰，至第7天阳性表达仍处高水平。结论 长时间冷保存降低了部分肝移植术后的肝再生能力和大鼠术后生存率。 【关键词】 部分肝移植； 肝再生； 冷保存； 大鼠 　　Effect of cold preservation on liver regeneration after partial liver transplantation in rats 【Abstract】 Objective To evaluate the effect of different duration of cold preservation on survival rate and liver regeneration after partial liver transplantation in rats. Methods All SD rats were divided into liver resection group (Group Ⅰ), partial liver transplantation plus 1 hour cold preservation group (Group Ⅱ) and partial liver transplantation plus 8 hours cold preservation group (Group Ⅲ). The 7day survival rate was observed. The liver/body weight ratio, liver regeneration rate, hepatocyte mitotic index and the expression of the proliferative cell nuclear antigen (PCNA) were compared among three groups at hour 1, 6, 12, 24, 48, 72 and 168 after operation. Results The 7day survival rate in Groups Ⅰ, Ⅱ and Ⅲ was 100%, 90% and 40%, respectively. The liver/body weight ratio, liver regeneration rate and hepatocyte mitotic index in Group Ⅲ were significantly lower than those in Groups Ⅰ and Ⅱ at postoperative days 2 and 3 (Plt;0.05). Compared with Groups Ⅰ and Ⅱ, the PCNA expression in Group Ⅲ was significantly lower within 12 hours after operation (Plt;0.05), reached peak at hour 48 and remained at a high level at day 7. Conclusion Long period of cold preservation significantly impairs the regenerative ability of the liver and survival rate of rats after partial liver transplantation. 【Key words】 Partial liver transplantation; Liver regeneration; Cold preservation; Rats 供肝短缺已成为影响肝移植发展的主要原因。部分肝移植作为解决这一问题的重要手段近年来得以广泛开展。供肝在移植过程中将不可避免地遭受冷保存损伤［1］，而部分肝移植的供肝还额外遭受了大块组织的丧失，它必须通过快速再生来确保其正