卡巴胆碱对大鼠烫伤休克肠内补液时肠血管通透性及组织水肿影响.docVIP

卡巴胆碱对大鼠烫伤休克肠内补液时肠血管通透性及组织水肿影响 　 作者：吴静，胡森，汪剑威，耿世佳，盛志勇 【摘要】 目的 研究烫伤休克大鼠肠内补液时给予卡巴胆碱对肠血管通透性及组织水肿的影响。方法健康雄性Wistar大鼠48只，随机分为单纯烫伤组(S组)、葡萄糖电解质组(GES组)、卡巴胆碱治疗组(CAR组)和葡萄糖-电解质+卡巴胆碱治疗组(GES/CAR组)，每组12只。制作35%休表面积(TBSA)Ⅲ°烫伤模型，肠内输入GES和(或)CAR(60μg/kg)进行复苏。应用改良伊文思蓝(EB)渗出法测定烫伤后4小时肠内补液时肠血管通透性及用干湿重法测定肠组织含水率的变化。结果 GES组与S组比较，肠组织EB含量显著增加(Plt;0.05)，肠组织含水率增加，但无统计学意义(Pgt;0.05)。给予卡巴胆碱治疗后，CAR组与GES/CAR组肠组织EB含量分别比S组与GES组显著下降(Plt;0.05)。CAR组比S组肠组织含水率减少(Plt;0.01)，GES/CAR组肠组织含水率比GES组显著减少(Plt;0.01)。结论 卡巴胆碱能降低烫伤休克口服补液时肠血管通透性，减轻肠黏膜组织水肿，对烫伤休克肠内补液时小肠缺血再灌注损伤具有保护作用。 【关键词】 烫伤;卡巴胆碱;肠内补液;血管通透性 Abstract： Objective To investigate the effects of carbachol on intestinal vascular permeability and tissue edema with enteral glucoseelectrolyte solution(GES) resuscitation in scald shock rats.Methods Wistar rats were randomly divided into four groups: scald alone (S,n=12),scald with enteral carbachol(60μg/kg,CAR,n=12),scald with enteral GES(GES,n=12) and scald with enteral GES containing 60μg/kg of carbachol(GES/CAR,n=12)groups.Rat model of 35% total body surface area(TBSA) Ⅲ degree scald model were reproduced.Evans blue(EB) and wetdry method were used to evaluate the intestinal microvascular permeability and tissue edema in each group.Results Intestinal vascular permeability in GES group(140.19±5.39)μg/g was significantly higher than that in S group (127.36±6.79)μg/g.There was significant difference(Plt;0.05).Intestinal water contents in GES group (82.283±1.277)% were higher than that in S group(79.525±2.119)%.Intestinal vascular permeability in CAR and GES/CAR group(73.32±5.34)μg/g and (105.63±4.53)μg/g was significantly lower than those in S and GES group(Plt;0.05). Intestinal water contents in CAR and GES/CAR group (76.114±4.654)% and (79.667±2.649)% were much lower than that in S and GES group(Plt;0.01).Conclusion Carbachol can significantly reduce the increase in intestinal microvascular permeability and water contents,and it might have protective effect on ischemia and reperfusion injury during enteral resusci