利脉胶囊对小鼠学习记忆影响.docVIP

利脉胶囊对小鼠学习记忆影响 　 作者：王帮华， 陈红光，赵骥，郑敏 【摘要】 目的 观察利脉胶囊对东莨菪碱致小鼠学习记忆障碍及正常小鼠空间学习记忆的影响。方法 采用跳台实验观察利脉胶囊（140mg／kg ig qd×14）对东莨菪碱所致小鼠学习记忆障碍的改善作用，以触电潜伏期及单位时间内错误次数作为学习记忆成绩；采用Morris水迷宫实验观察利脉胶囊（140mg／kg，ig qd×20）对正常小鼠空间学习记忆的影响，以定位航行实验小鼠逃避潜伏期及空间探索实验小鼠经过目标平台次数作为学习记忆成绩。结果 ①跳台试验。第 1d跳台训练，利脉胶囊组3min内跳台错误次数少于模型组 (Plt;0.01)，而和正常组无显著性差异（Pgt;0.05）。第2d跳台检测，东莨菪碱模型组触电潜伏期较正常对照组明显缩短；利脉胶囊治疗组触电潜伏期显著长于模型组（Plt;0.01),而与正常对照组接近，5min错误次数有减少趋势。②水迷宫试验。利脉胶囊治疗组逃避潜伏期小于正常组，经过目标平台次数有增加趋势。结论 利脉胶囊对东莨菪碱所致小鼠学习记忆障碍有一定的改善作用，并具有一定的促智作用。 【关键词】 利脉胶囊；东莨菪碱；跳台实验；学习记忆；Morris水迷宫实验 ABSTRACT:Objective To observe the effect of Limai capsule on learning and memory defict induced by scopolamine and spatial memory ability of normal mice.Methods The stepdown test and Morris water maze were adopted to observe the effect of Limai capsule (140 mg/kg, ig) on the learning and memory defict induced by scopolamine and spatial memory ability of normal mice respectively.Results ①On the first training day, the error times of Limai capsule group were less than those in model group within 3 minutes (Plt;0.01). No significant difference was found between Limai capsule group and nomal group（Pgt;0.05）. On the second detected day, the electrical injury latency in model group was less than that in normal group. Compared with model group, Limai capsule could significantly prolong the electrical injury latency and was close to the normal one（Plt;0.01). ②Compared with normal group, Limai capsule could significantly decrease the escape latency of normal mice but had no effect on the number of passing through primary flat place in Morris water maze test.Conclusion Limai capsule can improve learning and memory defict induced by scopolamine and has effect on intelligence increase. KEY WORDS: Limai capsule;Scopolamine;Learning and memory;Stepdown test;Morris water maze 老年痴呆是老年人致残最严重的疾病之一，主要表现为认知功能的下降和丧失。随着人口的老龄化，痴呆患者日益增多，给家庭和社会带来沉重的负担。因此寻找改善学习记忆障碍及具有促智作用的药物，成为当今国际医药界的重要研究课题。利脉胶囊每粒含阿魏酸钠 50mg、乙酰水杨酸(阿司匹林) 20mg、桂利嗪(脑嗌嗪)25mg、维生素B1 10mg。本品4种成分经组方后有协同作用，具有较