实时荧光PCR在男性性病尿道炎及男性尿道炎后综合征应用评价.docVIP

实时荧光PCR在男性性病尿道炎及男性尿道炎后综合征应用评价 　【摘要】 目的 探讨实时荧光PCR技术检测男性性病尿道炎及男性尿道炎后综合征(MPUS)患者的病原体的临床价值。方法 用实时荧光PCR法对718例男性性病尿道炎及MPUS患者尿道分泌物或前列腺液同时作淋球菌(NG)、沙眼衣原体(Ct)、解脲支原体(UU)检测。结果 52.2%的淋病患者并发Ct、UU感染。非淋菌性尿道炎(NGU)患者Ct、UU阳性率68.2%，Ct阳性率31.1%、UU阳性率42.6%，5.4%患者Ct、UU合并感染。MPUS患者NG、Ct、UU阳性率仅为10.2%。结论 实时荧光PCR技术灵敏度高，特异性强，对男性性病尿道炎及MPUS的诊断和治疗具有重要的指导意义。 【关键词】 男性性病尿道炎;男性尿道炎后综合征;病原体;实时荧光PCR 　　Abstract: Objective To discuss the clinical effectiveness of real-time PCR in detecting the pathogens of male′s sexually transmitted urethritis and male′s post-urethritis syndrome (MPUS). Methods Real-time PCR was used to detect the pathogens including Neisseria gonorrhoeae (NG), Chlamydia trachomatis (Ct) and Ureaplasma urealyticum (UU) in the urinary secretions or succus prostaticus from 718 male patients with sexually transmitted urethritis and/or post-urethritis sydrome. Results The prevalence of Ct and UU was 52.2% in the gonorrhoea patients and was 68.2% in the patients with nongonococcal urethritis (NGU). The positive rate was 31.1% for Ct and 42.6% for UU in NGU and the co-infection rate of Ct and UU was 5.4%, while the total prevalence of Ct, NG and UU was only 10.2% in the MPUS patients. Conclusion The technique of real-time PCR has very high sensitivity and specificity. It serves as significant guidance in the diagnosis and treatment of male′s sexually transmitted urethritis and MPUS. 　　Key words: male′s sexually transmitted urethritis; male′s post-urethritis syndrome; pathogen; real-time PCR 　　已换 男性性病尿道炎是常见的性传播疾病之一，其实验室诊断依据为检测患者尿道分泌物或前列腺液中的病原体，包括淋球菌(NG)、沙眼衣原体(Ct)、解脲支原体(UU)。目前，检测病原体方法很多，实时荧光PCR技术是比较敏感的方法。为评价实时荧光PCR技术在男性性病尿道炎及男性尿道炎后综合征(male′s post-urethritis sydrome，MPUS)诊治中的价值，我们对718例男性性病尿道炎及MPUS患者尿道分泌物或前列腺液同时检测NG、Ct、UU并进行分析。结果报道如下。 　　1 资料和方法 　　1.1 一般资料 718例男性性病尿道炎及MPUS患者均来自我院皮肤科门诊，其中男性性病尿道炎238例，MPUS 480例。718例患者均有不洁性交史，年龄18～62岁，平均38岁。男性性病尿道炎均为未经治疗的初诊患者，表现为尿频、尿急、尿道痒痛不适，有黄色分泌物或少量稀薄微浊分泌物或无分泌物。MPUS患者均经外院诊治过或过度治疗过而始终有尿频、尿急、尿痛等不适症状，病程3～12个月，外院实验室检查NG、Ct、UU均阴性(非实时荧光PCR法)。 　　1.2 样本采集 有尿道分泌物的患者取样：用无菌棉拭子粘取