川芎嗪对兔肾缺血再灌注损伤超微结构改变干预作用.docVIP

川芎嗪对兔肾缺血再灌注损伤超微结构改变干预作用 　【摘要】 目的:观察兔肾缺血再灌注损伤超微结构改变，探讨川芎嗪注射液的干预作用。方法:复制家兔肾缺血再灌注损伤模型，日本大耳白兔30只，随机均分为三组：假手术组（S），缺血再灌注组（IR）和缺血再灌注+川芎嗪注射液组（LZ）。使用透射电子显微镜观察各组肾组织标本超微结构的变化，作对比分析。结果:IR组主要异常改变有：①肾小球毛细血管腔狭窄，中性粒细胞附壁，大部分内皮细胞核固缩、染色体边集，细胞质内空泡形成，线粒体空泡变性。②多数脏层上皮细胞轻度核固缩，个别线粒体空泡变性，足突相互黏连，可见局灶性融合现象和微绒毛化现象。③近曲小管上皮细胞核固缩，胞质内出现较多空泡，溶酶体和致密颗粒沉积增多，线粒体数量减少，部分线粒体嵴断裂、空泡化，腔面微绒毛稀疏并有轻度肿胀现象。LZ组大部分标本的上述部位存在不同程度损伤性改变，但比IR组均明显减轻，其中近曲小管上皮细胞超微结构的改善较显著。结论:川芎嗪注射液可减轻肾缺血再灌注损伤所致的肾小球和肾小管超微结构的异常变化。 【关键词】 肾脏 缺血再灌注损伤 超微结构 川芎嗪注射液 Abstract: Objective: To observe the ultrastructural changes in renal tissue during renal ischemia reperfusion injury in rabbits and study the interfering effects of ligustrazine injection. Methods: The model of renal ischemia reperfusion injury of rabbit was established in vivo. Thirty rabbits were divided into three groups randomly: sham operation group (S group), ischemia reperfusion injury group (IR group) and ischemia reperfusion injury plus ligustrazine injection group (LZ group).The ultrastructural changes in renal tissue were observed under electron microscope in 5 hours after reperfusion. Results: The abnormal ultrastructure in IR group included that ①The cavities of the glomerular capillary narrowed, polymorphonuclear leucocytes adhered to the wall of the veessel, most of the endothelia showed nucleus pyknosis and staining thick, chromosome gathered near to nuclear membrance, vacuolization in cytoplasm and some mitochondia vacuolar degeneration were seen. ②Light degree nucleus pyknosis demonstrated in most of the visceral layer of the epithelia, individual mitochondrial vacuolar degeneration, foot process mutual adhesion were seen with focal coalescence and microvillus. ③epithelial cells of proximal convoluted tubule appeared nucleus pyknosis, more vacuolization was seen in cytoplasm, lysosome and dense granule in cytoplasm increased, number of mitochondrial vacuolization decreased, its crest partly fragmented, microvilli rarefaction and swelling were found. The ultrastructural