抗草甘膦基因aroAM12及抗虫基因Bts1m的转基因棉株.pdfVIP

31 1 Vol. 31, No. 1 2005 1 108~ 113 ACTA AGRONOMICA SINICA pp. 108- 113 Jan. , 2005 aroAM12 Bts1m 1 1,2 3 1,* 赵福永 谢龙旭 田颖川 徐培林 1 2 3 ( , 510275; , 53002; , 100080) : pAM12-s1m, ( gene shuffling) ( aroAM12 ) Bt ( Bts1m) aroAM12 CaMV35S , Bts1m 2E-35S 8 , aroAM12 Bts1m 321 , aroAM12 , 52 PCR Southern blot aroAM12 , 38 aroAM12 Bts1m Northern blotWestern blot , : ; ; aroAM12 ; Bts1m ; : S562 Glyphosate-resistant and Bollworm-resistant Transgenic Cotton Plants with the aroAM12 and Bts1m Genes 1 1, 2 3 1, * ZHAO Fu-Yong , XIE Long-Xu ,TIAN Ying-Chuan , XU Pe-i Lin 1 2 ( The Key Laboratory of Gene Engineering of Ministry of Education, Zhongshan University , Guang hou 510275, Guangdong ; College of Lfi e Science , Henan Nor- 3 mal University , Xinx iang 53002, Henan ; Institute of Microbiology , ChineseA cademy of Sciences, Beij ing 100080, China) Abstract:A new binary vector, pAM12-s1m, harboring the aroAM12 gene encoding 5-enolpyruvyl-shikimate-3-phosphate synthase ( EPSPS) and a synthetic recombinant Bts1m toxin gene consisting of 331N-terminal amino acids of CryIAc and 28 C-terminal amino acids of CryIAb was constructed1 The truncated aroAM12 gene, which was obtained through gene shuffling technology, was ligated with a transit sequence of Arabidopsis EPSPS and expressed in cotton plants, and driven by cauliflower mosaic virus 35S ( CaMV35S) promoter1 T