the potential biotechnological applications of the exopolysaccharide produced by the halophilic bacterium halomonas almeriensis潜在的嗜盐细菌产生的胞外多糖的生物技术应用halomonas almeriensis.pdfVIP

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the potential biotechnological applications of the exopolysaccharide produced by the halophilic bacterium halomonas almeriensis潜在的嗜盐细菌产生的胞外多糖的生物技术应用halomonas almeriensis.pdf

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the potential biotechnological applications of the exopolysaccharide produced by the halophilic bacterium halomonas almeriensis潜在的嗜盐细菌产生的胞外多糖的生物技术应用halomonas almeriensis

Molecules 2012, 17, 7103-7120; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article The Potential Biotechnological Applications of the Exopolysaccharide Produced by the Halophilic Bacterium Halomonas almeriensis Inmaculada Llamas, Hakima Amjres, Juan Antonio Mata, Emilia Quesada and Victoria Béjar * Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain; E-Mails: illamas@ugr.es (I.L.); amjresha@yahoo.fr (H.A.); jonanmg@ (J.A.M.); equesada@ugr.es (E.Q.) * Author to whom correspondence should be addressed; E-Mail: vbejar@ugr.es; Tel.: +34-958-241-705; Fax: +34-958-216-035. Received: 31 March 2012; in revised form: 31 May 2012 / Accepted: 5 June 2012 / Published: 12 June 2012 Abstract: We have studied the extracellular polysaccharide (EPS) produced by the type strain, M8T, of the halophilic bacterium Halomonas almeriensis, to ascertain whether it might have any biotechnological applications. All the cultural parameters tested influenced both bacterial growth and polysaccharide production. EPS production was mainly growth-associated and under optimum environmental and nutritional conditions M8T excreted about 1.7 g of EPS per litre of culture medium (about 0.4 g of EPS per gram of dry cell weight). Analysis by anion-exchange chromatography and high-performance size-exclusion