the production of extracellular proteins is regulated by ribonuclease iii via two different pathways in staphylococcus aureus细胞外蛋白质的生产是由核糖核酸酶iii在金黄色葡萄球菌通过两种不同的途径.pdfVIP

The Production of Extracellular Proteins Is Regulated by Ribonuclease III via Two Different Pathways in Staphylococcus aureus Yu Liu., Jie Dong., Na Wu, Yaping Gao, Xin Zhang, Chunhua Mu, Ningsheng Shao, Ming Fan, Guang Yang* Beijing Institute of Basic Medical Sciences, Beijing, People’s Republic of China Abstract Staphylococcus aureus ribonuclease III belongs to the enzyme family known to degrade double-stranded RNAs. It has previously been reported that RNase III cannot influence cell growth but regulates virulence gene expression in S. aureus. Here we constructed an RNase III inactivation mutant (Drnc) from S. aureus 8325-4. It was found that the extracellular proteins of Drnc were decreased. Furthermore, we explored how RNase III regulated the production of the extracellular proteins in S. aureus. We found during the lag phase of the bacterial growth cycle RNase III could influence the extracellular protein secretion via regulating the expression of secY2, one component of accessory secretory (sec) pathway. After S. aureus cells grew to exponential phase, RNase III can regulate the expression of extracellular proteins by affecting the level of RNAIII. Further investigation showed that the mRNA stability of secY2 and RNAIII was affected by RNase III. Our results suggest that RNase III could regulate the pathogenicity of S. aureus by influencing the level of extracellular proteins via two different ways respectively at different growth phases. Citation: Liu Y, Dong J, Wu N, Gao Y, Zhang X, et al. (2011) The Production of Extracellular Proteins Is Regulated by Ribonuclease III via Two Different Pathways in Staphylococcus aureus. PLoS ONE 6(5): e20554. doi:10.1371/journal.pone.0020554 Editor: J. Ross Fitzgerald, University of Edinburgh, United Kingdom Received December 6, 2010; Accepted May 5, 2011; Publish